Artículo de revista
Isolation of a novel complex of the SWI/SNF family from Schizosaccharomyces pombe and its effects on in vitro transcription in nucleosome arrays
Fecha
2007-09Registro en:
MOLECULAR AND CELLULAR BIOCHEMISTRY,v.: 303, issue: 1-2, p.: 131-139, SEP 2007
0300-8177
Autor
Bernal, Giuliano
Maldonado Maldonado, Edio
Institución
Resumen
The family of ATP-dependent chromatinremodeling
factors plays a central role in eukaryotic
transcriptional regulation. These complexes can alter the
structure of chromatin by mechanisms that involve
nucleosome sliding, dissociation, or replacement over a
specific promoter. The SWI/SNF chromatin-remodeling
complex is required for transcriptional activation or
repression in a subset of genes. In the present study we
have isolated the spSWI/SNF complex from Schizosaccharomyces
pombe, which has at least seven subunits
among them spSwi1-like and the catalytic subunit spBrg1.
These subunits are homologues to Swi1 and Swi2/Snf2,
respectively in Sacharomyces cerevisiae. Moreover, we
have demonstrated that spSWI/SNF is able to promote
in vitro transcription by RNA polymerase II (RNAPII) in
a reconstituted system. In our transcription assays with
cellular extracts of Sc. pombe we did not observe inhibition
when a-Swi1 antibodies were utilized, indicating
that other chromatin-remodeling complexes may allow
transcription in Sc. pombe.