| dc.description.abstract | Osteoporosis is characterized by low bone mass, microarchitectural deterioration of bone tissue leading to increased bone fragility, and a
resulting susceptibility to fractures. Distinctive environmental bone marrow conditions appear to support the development and
maintenance of the unbalance between bone resorption and bone formation; these complex bone marrow circumstances would be
reflected in the fluid surrounding bone marrow cells. The content of regulatory molecules in the extracellular fluid from the human bone
marrow is practically unknown. Since the content of cytokines such as adiponectin, leptin, osteoprogeterin (OPG), soluble receptor
activator of nuclear factor kB ligand (s-RANKL), tumor necrosis factor a, and interleukin 6 (IL-6) may elicit conditions promoting or
sustaining osteoporosis, in this work we compared the concentrations of the above-mentioned cytokines and also the level of the soluble
receptors for both IL-6 and leptin in the extracellular fluid from the bone marrow of nonosteoporotic and osteoporotic human donors.
A supernatant fluid (bone marrow supernatant fluid [BMSF]) was obtained after spinning the aspirated bone marrow samples; donors
were classified as nonosteoporotic or osteoporotic after dual-energy X-ray absorptiometry (DXA) measuring. Specific commercially
available kits were used for all measurements. The cytokines’ concentration in BMSF showed differently among nonosteoporotic
and osteoporotic women; this last group was characterized by higher content of proinflammatory and adipogenic cytokines. Also,
osteoporotic BMSF differentiated by decreased leptin bioavailability, suggesting that insufficient leptin action may distinguish the
osteoporotic bone marrow. | |
