Artículos de revistas
Synthesis and In Vivo Evaluation of the Biodistribution of a 18FLabeled Conjugate Gold-Nanoparticle-Peptide with Potential Biomedical Application
Fecha
2012Registro en:
Bioconjugate Chemistry. 2012, 23, 399−408. DOI: dx.doi.org/10.1021/bc200362a
1520-4812
Autor
Guerrero, Simon
Herance, José Raul
Rojas, Santiago
Mena, Juan F.
Gispert, Juan Domingo
Acosta, Gerardo A.
Albericio, Fernando
Kogan Bocian, Marcelo
Institución
Resumen
Gold nanoparticles (AuNPs) have been extensively used in biological applications because of their
biocompatibility, size, and ease of characterization, as well as an extensive knowledge of their surface chemistry. These features
make AuNPs readily exploitable for biomedical applications, including drug delivery and novel diagnostic and therapeutic
approaches. In a previous work, we studied ex vivo distribution of the conjugate C(AuNP)-LPFFD for its potential uses in the
treatment of Alzheimer’s disease. In this study, we covalently labeled the conjugate with [18F]-fluorobenzoate to study the in vivo
distribution of the AuNP by positron emission tomography (PET). After intravenous administration in rat, the highest
concentration of the radiolabeled conjugate was found in the bladder and urine with a lower proportion in the intestine,
demonstrating progressive accumulation compatible with biliary excretion of the conjugate. The conjugate also accumulated in
the liver and spleen. PET imaging allowed us to study the in vivo biodistribution of the AuNPs in a noninvasive and sensitive way
using a reduced number of animals. Our results show that AuNPs can be covalently and radioactively labeled for PET
biodistribution studies.