Artículos de revistas
Osmotically-induced genes are controlled by the transcription factor TonEBP in cultured cardiomyocytes
Fecha
2008-07-25Registro en:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Volume: 372, Issue: 2, Pages: 326-330 : JUL 25 2008
0006-291X
Autor
Navarro, Paola
Chiong Lay, Mario
Volkwein, Karen
Moraga, Francisco
Ocaranza, María Paz
Jalil Milad, Jorge
Sun, Woo Lim
Jeong-Ah, Kim
H., Moo Kwon
Lavandero González, Sergio
Institución
Resumen
Changes in cardiac osmolarity occur in myocardial infarction. Osmoregulatory mechanisms may, therefore,
play a crucial role in cardiomyocyte survival. Tonicity-responsive enhancer binding protein
(TonEBP) is a key transcription factor participating in the adaptation of cells to increases in tonicity. However,
it is unknown whether cardiac TonEBP is activated by tonicity. Hypertonicity activated transcriptional
activity of TonEBP, increased the amounts of both TonEBP mRNA and protein, and induced both
the mRNA and protein of TonEBP target genes (aldose reductase and heat shock protein-70). Hypotonicity
decreased the amount of TonEBP protein indicating bidirectional osmoregulation of this transcription factor.
Adenoviral expression of a dominant negative TonEBP suppressed the hypertonicity-dependent
increase of aldose reductase protein. These results indicated that TonEBP controls osmoregulatory mechanisms
in cardiomyocytes.