dc.creatorGreiner Gebauer, Monika Marcela
dc.creatorCárdenas Muñoz, Sergio Patricio
dc.creatorParra, C.
dc.creatorBravo, Jaime F.
dc.creatorAvalos, Ana María
dc.creatorParedes Vargas, Alfonso
dc.creatorLara Peñaloza, Hernán
dc.creatorFiedler Temer, Jenny
dc.date.accessioned2009-06-22T14:32:30Z
dc.date.accessioned2019-04-25T23:28:09Z
dc.date.available2009-06-22T14:32:30Z
dc.date.available2019-04-25T23:28:09Z
dc.date.created2009-06-22T14:32:30Z
dc.date.issued2001-08
dc.identifierENDOCRINE 15(3):323-333
dc.identifier0969-711X
dc.identifierhttp://repositorio.uchile.cl/handle/2250/120699
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/2425054
dc.description.abstractMorphological studies of granular neurons of the hippocampus have shown that adrenalectomy (ADX) induces the cell death of granular neurons, an effect prevented by corticosterone replacement. We addressed the hypothesis that corticosterone regulates the expression of the apoptotic bcl-2 gene family. Five days after adrenalectomy, we observed morphological changes related to hippocampal granule cell apoptosis that was accompanied by terminal dUTP nick and labeling (TUNEL) labeling in nuclei located in the hilus region. Corticosterone replacement prevented the cell death induced by ADX. Using RT-PCR we found a reduction in mRNA levels of the antiapoptotic gene bcl-2 in whole hippocampus, an effect which was prevented by corticosterone administration to ADX rats. However, Bcl-2 protein levels were not altered by this treatment. We did not observe modifications in the level of bcl-X-L mRNA however, we did find a 40% reduction in Bcl-X-L protein levels, an effect not reversed by corticosterone. In contrast, we found a reduction in the mRNA of the antiapoptotic gene bax and Bax levels after ADX; both effects were prevented by corticosterone. The reduction in proapoptotic bax and in antiapoptotic bcl-2 mRNA levels in the whole hippocampus, suggests that local variations in these molecules could account for both neuronal viability of the CA1-CA3 and granular cell death detected by morphological means and observed after ADX.
dc.languageen
dc.publisherHUMANA PRESS INC
dc.subjectAdrenalectomy
dc.titleAdrenalectomy regulates apoptotic-associated genes in rat hippocampus
dc.typeArtículos de revistas


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