UV–vis, IR and 1H NMR spectroscopic studies and characterization of ionic-pair crystal violet–oxytetracycline

Abstract

The present study shows the formation and characterization of the ionic-pair between the antibiotic oxytetracycline and the dye crystal violet in ammonia solutionpH9.0±0.2 extracted into chloroform. The characterization was demonstrated using UV–vis spectrophotometry, 1H NMR, measurement of relaxation times T1 and IR spectroscopy, using a comparison between the signals of individual pure compounds with the signals with the mixture CV–OTC in different alkaline media. The formation of ionic-pair was also corroborated by new signals and chemical shifts. (2D) NMR spectroscopy experiments show that the interaction is electrostatic.