Respuesta de las células HeLa y EA.hy926 al estrés por hipoxia y radiación ionizante a través de la vía JAK/STAT y la supervivencia celular

Abstract

The biological behavior of cancer cells is influenced by the microenvironment in which they develop and factors like angiogenesis and stressor stimuli such as hypoxia are considered critical for tumor development and therapeutic management. One of the molecular mechanisms involved in cellular response to stressful stimuli is the activation of intracellular signaling pathways, in this study we have evaluated the state of the JAK / STAT and therein the expression / activation of STAT3 protein in the tumor (HeLa) and endothelial (EA.hy926) cell lines subjected to physical and chemical hypoxia with deferoxamine mesylate for 2, 6 and 24 hours. Additionally, considering the importance of hypoxia as a modifying agent in the response of cancer management using ionizing radiation; cell survival curves were constructed to assess the cellular behavior compared to these stimuli. This study highlights the importance of hypoxia as a stimulus which modifies the STAT3 protein activation and survival of cells irradiated in the two cell lines studied.