Tesis
Microencapsulación de un bioinsumo obtenido a partir del beneficio de fique (Furcraea andina) para el manejo del hongo Botrytis cinerea
Autor
Barragan Montoya, Kewin Andrés
Villamizar Vergara, Oscar Duvan
Institución
Resumen
A microencapsulated bioinsumption was prepared, obtained by means of treatments to
benefit the fique (Furcraea Andina) and the antimicrobial properties were evaluated in
the Biosystems Center of the Jorge Tadeo Lozano University. Evaluating the control over
the fungus Botrytis cinerea, phytopathogenic species capable of attacking more than 200
types of plants and great problem in agriculture; the effectiveness of microencapsulated
bioinsumption was compared, in different proportions (alginate:extract) and with different
extract solutions (aqueous & ethanolic). The growth control measurements were made
for ten days, by in vitro tests, taking into account the amount by weight of the
microcapsules (aqueous extract and ethanolic extract) in the medium (PDA). A physical,
chemical and biological characterization was performed for the microcapsules, where an
average diameter of between 1.320 mm - 2.040 mm was obtained, this depending on
the diameter of the needle used in the microencapsulator. The structure of the aqueous
microcapsules has different morphology in relation to the ethanolic microcapsules, this
is identified by scanning electron microscopy (SEM), chemically evidenced by infrared
spectroscopy by Fourier transform (FT-IR), difference in the peak at the length
comprised of 1400 cm-1 to 1000 cm-1 for aqueous microcapsules and ethanolic
microcapsules, which reflects the insolubility of cellulose with water, thus giving the
greatest difference between aqueous microcapsules with respect to ethanolic
microcapsules. Said procedures were carried out with the purpose of establishing the
viability of the development of microcapsules of extract (aqueous and / or ethanolic) for
the control of the fungus Botrytis, having through the in vitro tests, there was an alteration
in its normal growth using the microcapsules of extract aqueous, whereas, for the
microcapsules made with the ethanol extract, the best percentage of inhibition is
presented for the T1 treatment, with a value of 12.5%.