Artículos de revistas
Sequential depletion of rat testicular lipids with long-chain and very long-chain polyenoic fatty acids after X-ray-induced interruption of spermatogenesis
Fecha
2010-09Registro en:
Oresti, Gerardo Martin; Ayuza Aresti, Pablo Leonardo; Gigola, Graciela; Reyes, Luis E.; Aveldaño, Marta Isabel; Sequential depletion of rat testicular lipids with long-chain and very long-chain polyenoic fatty acids after X-ray-induced interruption of spermatogenesis; American Society for Biochemistry and Molecular Biology; Journal of Lipid Research Papers In Press; 51; 9; 9-2010; 2600-2610
0022-2275
1539-7262
CONICET Digital
CONICET
Autor
Oresti, Gerardo Martin
Ayuza Aresti, Pablo Leonardo
Gigola, Graciela
Reyes, Luis E.
Aveldaño, Marta Isabel
Resumen
When a single dose of X-rays is applied to the adult rat testis, stem spermatogonia are damaged and spermatogenesis is interrupted. Supported by Sertoli cells, those spermatogenic cells that endure irradiation complete their differentiation and gradually leave the testis as spermatozoa. In this study, the in vivo changes taking place a number of weeks after irradiation revealed cell-specific features of testicular lipid classes. A linear drop, taking about 6 weeks, in testis weight, non-lipid materials, free cholesterol and 22:5n-6-rich glycerophospholipids took place with germ cell depletion. Sphingomyelins and ceramides with non-hydroxy very long chain polyenoic fatty acids (n-VLCPUFA) disappeared in 4 weeks, together with the last spermatocytes, whereas species with 2-hydroxy VLCPUFA lasted for 6 weeks, disappearing with the last spermatids and spermatozoa. The amount per testis of 22:5n-6-rich triacylglycerols, unchanged for 4 weeks, fell between weeks 4-6, associating these lipids with spermatids and their residual bodies, detected as small bright lipid droplets. In contrast, 22:5n-6-rich species of cholesterol esters, and large lipid droplets, increased in seminiferous tubules up to week 6, revealing they are Sertoli cell products. At week 30, the lipid and fatty acid profiles reflected the resulting permanent testicular involution. Our data highlight the importance of Sertoli cells in maintaining lipid homeostasis during normal spermatogenesis.