info:eu-repo/semantics/article
Neutrophils suppress γδ T-cell function
Fecha
2013-12Registro en:
Sabbione, Florencia; Gabelloni, María Laura; Ernst, Glenda; Gori, María Soledad; Salamone, Gabriela Veronica; et al.; Neutrophils suppress γδ T-cell function; Wiley VCH Verlag; European Journal of Immunology; 44; 3; 12-2013; 819-830
0014-2980
CONICET Digital
CONICET
Autor
Sabbione, Florencia
Gabelloni, María Laura
Ernst, Glenda
Gori, María Soledad
Salamone, Gabriela Veronica
Oleastro, Matías
Trevani, Analía Silvina
Geffner, Jorge Raúl
Jancic, Carolina Cristina
Resumen
γδ T cells have been shown to stimulate the recruitment and activation of neutrophils through the release of a range of cytokines and chemokines. Here, we investigated the reverse relationship, showing that human neutrophils suppress the function of human blood γδ T cells. We show that the upregulation of CD25 and CD69 expression, the production of IFN-γ, and the proliferation of γδ T cells induced by (E)-1-hydroxy-2-methylbut-2-enyl 4-diphosphate are inhibited by neutrophils. Spontaneous activation of γδ T cells in culture is also suppressed by neutrophils. We show that inhibitors of prostaglandin E2 and arginase I do not exert any effect, although, in contrast, catalase prevents the suppression of γδ T cells induced by neutrophils, suggesting the participation of neutrophil-derived ROS. We also show that the ROS-generating system xanthine/xanthine oxidase suppresses γδ T cells in a similar fashion to neutrophils, while neutrophils from chronic granulomatous disease patients only weakly inhibit γδ T cells. Our results reveal a bi-directional cross-talk between γδ T cells and neutrophils: while γδ T cells promote the recruitment and the activation of neutrophils to fight invading pathogens, neutrophils in turn suppress the activation of γδ T cells to contribute to the resolution of inflammation.