Artículos de revistas
TNF promoter SNP variation in Amerindians and white-admixed women from Misiones, Argentina
Fecha
2013-06Registro en:
Badano, Ines; Schurr, T. G.; Stietz, S. M.; Dulik, M. C.; Mampaey, M.; et al.; TNF promoter SNP variation in Amerindians and white-admixed women from Misiones, Argentina; Wiley; International Journal of Immunogenetics; 40; 3; 6-2013; 216-221
1744-313X
Autor
Badano, Ines
Schurr, T. G.
Stietz, S. M.
Dulik, M. C.
Mampaey, M.
Quintero, I. M.
Zinovich, J. B.
Campos, Rodolfo Hector
Liotta, Domingo Javier
Resumen
The aim of this study is to describe genetic variation in the TNF promoter in the ethnically diverse population of Misiones, north-eastern Argentina. We analysed 210 women including 66 Amerindians of the Mbya-Guarani ethnic group and 144 white-admixed individuals from urban and rural areas of Misiones. Their DNA samples were surveyed for TNF polymorphisms -376 A/G, -308 A/G -244 A/G and -238 A/G by PCR amplification and direct sequencing and for the Amerindian marker -857 C/T by real-time PCR. Our main findings are as follows:(i) a distinctive pattern of Single Nucleotide Polymorphism (SNP) distribution among these groups, (ii) genetic differentiation between the Mbya-Guarani and the white-admixed populations (P < 0.05), (iii) lower gene diversity (~0.05) in Mbya-Guarani compared with the whiteadmixed group (~0.21); and (iv) linkage disequilibrium between the -376A and -238A SNPs in white-admixed populations. These data highlight the principal role of population history in establishing present-day genetic variation at the TNF locus and provide a framework for undertaking ethnographic and disease association studies in Misiones.