Asymmetric hydrolysis of dimethyl 3-phenyl glutarate in sequential batch reactor operation catalyzed by immobilized Geobacillus thermocatenulatus lipase

Abstract

The asymmetric hydrolysis of dimethyl 3-phenyl glutarate catalyzed by different immobilized preparations of Geobacillus thermocatenulatus lipase (BTL2) in sequential batch reactor operation was studied. Modified sepharose and silica supports were used for the immobilization of BTL2 using different strategies. The lipase immobilized on silica heterofunctional support (Silica-HE) had the highest activity expressed (68 UI g-1), however the BTL2 immobilized on sepharose heterofunctional (Seph-HG) support was selected for the asymmetric hydrolysis in sequential batch reactor operation, because its reaction rate (0.0216 μmole P/g biocatalysts h) and enantiomeric excess (ee) (82%) were higher than others biocatalysts. During the first two batch the ee remained, in the third batch the ee decreased possibly by a change in the conformation of the enzyme. Key-words. Asymmetric hydrolysis, dimethyl 3-phenyl glutarate, sepharose supports, silica supports, sequential batch reactor, heterofunctional support.