dc.date.accessioned2018-09-03T11:46:49Z
dc.date.available2018-09-03T11:46:49Z
dc.date.created2018-09-03T11:46:49Z
dc.date.issued2017
dc.identifierhttp://hdl.handle.net/10533/219692
dc.identifier1130835
dc.identifierWOS:000411985200057
dc.description.abstractPlatelets have a major role in clotting activation and contribute to the innate immune response during systemic infections. Human platelets contain tissue factor (TF) and express functional Toll-like receptor 4 (TLR4). However, the role of TLR4 in triggering the procoagulant properties of platelets, upon challenge with bacteria, is yet unknown. Our hypothesis is that E. coli O111-TLR4 interaction activates platelets and elicits their procoagulant activity. We demonstrated that the strain, but not ultrapure LPS, increased surface P-selectin expression, platelet dependent TF procoagulant activity (TF-PCA) and prompted a faster thrombin generation (TG). Blockade of TLR4 resulted in decreased platelet activation, TF-PCA and TG, revealing the participation of this immune receptor on the procoagulant response of platelets. Our results provide a novel mechanism by which individuals with bacterial infections would have an increased incidence of blood clots. Furthermore, the identification of platelet TF and TLR4 as regulators of the effect of E. coli O111 might represent a novel therapeutic target to reduce the devastating consequences of the hemostatic disorder during sepsis. Keywords. KeyWords Plus:HEPARIN-INDUCED THROMBOCYTOPENIA; MESSENGER-RNA; BACTERIAL LPS; SHIGA-TOXIN; IN-VIVO; ACTIVATION; EXPRESSION; INFECTION; INFLAMMATION; THROMBIN
dc.languageeng
dc.relationhttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0185431
dc.relation10.1371/journal.pone.0185431
dc.relationinfo:eu-repo/grantAgreement//1130835
dc.relationinfo:eu-repo/semantics/dataset/hdl.handle.net/10533/93477
dc.relationinstname: Conicyt
dc.relationreponame: Repositorio Digital RI2.0
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
dc.titleHuman platelet interaction with E-coli O111 promotes tissue-factor-dependent procoagulant activity, involving Toll like receptor 4
dc.typeArticulo


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