Actas de congresos
20S Proteasome: Structural study using SAXS.
Fecha
2013-02-26Registro en:
Reunião Anual de Usuários do LNLS RAU, XXIII, 2013, Campinas
Autor
Bicev, Renata Naporano
Oliveira, Cristiano Luis Pinto de
Demasi, M.
Institución
Resumen
The process of intracellular proteolysis (protein degradation) is a regulatory mechanism of cellular homeostasis with the same level of importance as gene expression.The proteasome is a proteolytic complex responsible for protein degradation
and consists of a catalytic core unit called the 20S(20SPT) where the hydrolysis
occurs, engaged in one or both ends by regulatory units, called 19S, responsible
for the recognition of poly-ubiquitylated proteins, unfolding and translocation of
them to the 20S catalytic chamber. However, the catalytic unit (20SPT) can also
degrade not marked proteins with poly-ubiquitin tail, as in the case of oxidized
proteins. Oxidized proteins have a tendency to form aggregates (a phenomenon
that underlies human neurodegenerative diseases), and therefore they must be effectively removed from the living cell. Interestingly, the cells have approximately
1/3 of proteasome without regulatory units, i.e. only the 20S catalytic unit.