Artículos de revistas
Simultaneous quantification of lycopene, β-carotene, retinol and α -tocopherol in plasma after a simple extraction procedure: stability study and application to human volunteers
Fecha
2012Registro en:
J. Braz. Chem. Soc.,v.23,n.8,p.1441-1449,2012
0103-5053
10.1590/S0103-50532012005000001
Autor
Charão, Mariele F.
Moro, Angela M.
Brucker, Natália
Bulcão, Rachel P.
Baierle, Marília
Freitas, Fernando
Durgante, Juliano
Nascimento, Sabrina
Bubols, Guilherme B.
Saldiva, Paulo H.
Bohrer, Denise
Garcia, Solange C.
Institución
Resumen
A method for the simultaneous quantification of lycopene, β-carotene, retinol and α-tocopherol by high-performance liquid chromatography (HPLC) with Vis/fluorescence detection with isocratic elution was optimized and validated. The method consists of a rapid and simple liquid-liquid extraction procedure and a posterior quantification of extracted supernatants by HPLC. Aliquots of plasma were stored at -20°C for three months for stability study. The methodology was applied to samples from painters and individuals not exposed to paints (n = 75). The assay was linear for all vitamins (r > 0.99). Intra- and inter-run precisions were obtained with coefficient of variation smaller than 5%. The accuracies ranged from 0.29 to -5.80% and recoveries between 92.73 and 101.97%. Plasma samples and extracted supernatants were stable for 60 days at -20°C. A significant decrease of lycopene, β-carotene and retinol concentrations in plasma from exposed individuals compared to non-exposed individuals (p < 0.05) was observed. The method is simple, reproducible, precise, accurate and sensitive, and can be routinely utilized in clinical laboratories.