Artículos de revistas
Evidence of 5-aminolevulinic acid (ALA) penetration increase due to microdrilling in soft tissue using femtosecond laser ablation
Fecha
2012Registro en:
LASERS IN MEDICAL SCIENCE, LONDON, v. 27, n. 5, supl. 1, Part 2, pp. 1067-1071, SEP, 2012
0268-8921
10.1007/s10103-012-1051-1
Autor
Nicolodelli, Gustavo
Kurachi, Cristina
Rego, Raquel Ferreira
Omairi, Tarek
Bagnato, Vanderlei Salvador
Institución
Resumen
Photodynamic therapy (PDT) is a therapeutic technique mainly applied to the treatment of malignant and pre-malignant lesions, which induces cell death by the combined effect of a photosensitizer, irradiation in a proper wavelength, and molecular oxygen. One of the main limitations of PDT using 5-aminolevulinic acid (ALA) is the superficial volume of treatment, mainly due to the limited penetration of topical photosensitization. In this context, the present study investigates if a laser micromachining producing microchannels on the tissue surface could improve ALA penetration and result in an increase in the treatment depth. The laser micromachining under femtosecond regime was performed on the tissue surface of rat livers. Conventional PDT was applied and the induced depth of necrosis with or without laser micromachining was compared. The results showed an increase of more than 20% in the depth of necrosis when the femtosecond laser micromachining was performed before the treatment with the PDT.