Artículos de revistas
The defensive functions of plant inhibitors are not restricted to insect enzyme inhibition
Fecha
2010Registro en:
PHYTOCHEMISTRY, v.71, n.2/Mar, p.214-220, 2010
0031-9422
10.1016/j.phytochem.2009.10.009
Autor
SUMIKAWA, Joana Tomomi
BRITO, Marlon Vilela de
MACEDO, Maria Ligia Rodrigues
UCHOA, Adriana F.
MIRANDA, Antonio
ARAUJO, Ana Paula Ulian de
SILVA-LUCCA, Rosemeire A.
SAMPAIO, Misako Uemura
OLIVA, Maria Luiza Vilela
Institución
Resumen
Three plant proteinase inhibitors BbKI (kallikrein inhibitor) and BbCI (cruzipain inhibitor) from Bauhinia bouhinioides, and a BrTI (trypsin inhibitor) from B. rufa, were examined for other effects in Callosobruchus maculatus development; of these only BrTI affected bruchid emergence. BrTI and BbKI share 81% identities in their primary sequences and the major differences between them are the regions comprising the RGD and RGE motifs in BrTI. These sequences were shown to be essential for BrTI insecticidal activity, since a modified BbKI [that is a recombinant form (BbKIm) with some amino acid residues replaced by those found in BrTI sequence] also strongly inhibited insect development. By using synthetic peptides related to the BrTI sequence, YLEAPVARGDGGLA-NH(2) (RGE) and IVYYPDRGETGL-NH(2) (RGE), it was found that the peptide with an RGE sequence was able to block normal development of C. maculatus larvae (ED(50) 0.16% and LD(50) 0.09%), this being even more effective than the native protein. (C) 2009 Elsevier Ltd. All rights reserved.