Artículos de revistas
Immunization of neonatal mice with LAMP/p55 HIV gag DNA elicits robust immune responses that last to adulthood
Fecha
2010Registro en:
VIROLOGY, v.406, n.1, p.37-47, 2010
0042-6822
10.1016/j.virol.2010.06.050
Autor
RIGATO, Paula Ordonhez
MACIEL JR., Milton
GOLDONI, Adriana Leticia
PIUBELLI, Orlando
BRITO, Cyro Alves de
FUSARO, Ana Elisa
ALENCAR, Liciana Xavier Eurico de
AUGUST, Thomas
MARQUES JR., Ernesto Torres Azevedo
DUARTE, Alberto Jose da Silva
SATO, Maria Notomi
Institución
Resumen
Successful T cell priming in early postnatal life that can generate effective long-lasting responses until adulthood is critical in HIV vaccination strategies because it prevents early sexual initiation and breastfeeding transmission of HIV. A chimeric DNA vaccine encoding p55 HIV gag associated with lysosome-associated membrane protein 1 (LAMP-1; which drives the antigen to the MIIC compartment), has been used to enhance cellular and humoral antigen-specific responses in adult mice and macaques. Herein, we investigated LAMP-1/gag vaccine immunogenicity in the neonatal period in mice and its ability to generate long-lasting effects. Neonatal vaccination with chimeric LAMP/gag generated stronger Gag-specific immune responses, as measured by the breadth of the Gag peptide-specific IFN-gamma, proliferative responsiveness, cytokine production and antibody production, all of which revealed activation of CD4+ T cells as well as the generation of a more robust CTL response compared to gag vaccine alone. To induce long-lived T and B cell memory responses, it was necessary to immunize neonates with the chimeric IAMP/gag DNA vaccine. The LAMP/gag DNA vaccine strategy could be particularly useful for generating an anti-HIV immune response in the early postnatal period capable of inducing long-term immunological memory. (C) 2010 Elsevier Inc. All rights reserved.