dc.creator | RIGATO, Paula Ordonhez | |
dc.creator | MACIEL JR., Milton | |
dc.creator | GOLDONI, Adriana Leticia | |
dc.creator | PIUBELLI, Orlando | |
dc.creator | BRITO, Cyro Alves de | |
dc.creator | FUSARO, Ana Elisa | |
dc.creator | ALENCAR, Liciana Xavier Eurico de | |
dc.creator | AUGUST, Thomas | |
dc.creator | MARQUES JR., Ernesto Torres Azevedo | |
dc.creator | DUARTE, Alberto Jose da Silva | |
dc.creator | SATO, Maria Notomi | |
dc.date.accessioned | 2012-10-19T17:53:18Z | |
dc.date.accessioned | 2018-07-04T15:10:05Z | |
dc.date.available | 2012-10-19T17:53:18Z | |
dc.date.available | 2018-07-04T15:10:05Z | |
dc.date.created | 2012-10-19T17:53:18Z | |
dc.date.issued | 2010 | |
dc.identifier | VIROLOGY, v.406, n.1, p.37-47, 2010 | |
dc.identifier | 0042-6822 | |
dc.identifier | http://producao.usp.br/handle/BDPI/22749 | |
dc.identifier | 10.1016/j.virol.2010.06.050 | |
dc.identifier | http://dx.doi.org/10.1016/j.virol.2010.06.050 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1619480 | |
dc.description.abstract | Successful T cell priming in early postnatal life that can generate effective long-lasting responses until adulthood is critical in HIV vaccination strategies because it prevents early sexual initiation and breastfeeding transmission of HIV. A chimeric DNA vaccine encoding p55 HIV gag associated with lysosome-associated membrane protein 1 (LAMP-1; which drives the antigen to the MIIC compartment), has been used to enhance cellular and humoral antigen-specific responses in adult mice and macaques. Herein, we investigated LAMP-1/gag vaccine immunogenicity in the neonatal period in mice and its ability to generate long-lasting effects. Neonatal vaccination with chimeric LAMP/gag generated stronger Gag-specific immune responses, as measured by the breadth of the Gag peptide-specific IFN-gamma, proliferative responsiveness, cytokine production and antibody production, all of which revealed activation of CD4+ T cells as well as the generation of a more robust CTL response compared to gag vaccine alone. To induce long-lived T and B cell memory responses, it was necessary to immunize neonates with the chimeric IAMP/gag DNA vaccine. The LAMP/gag DNA vaccine strategy could be particularly useful for generating an anti-HIV immune response in the early postnatal period capable of inducing long-term immunological memory. (C) 2010 Elsevier Inc. All rights reserved. | |
dc.language | eng | |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | |
dc.relation | Virology | |
dc.rights | Copyright ACADEMIC PRESS INC ELSEVIER SCIENCE | |
dc.rights | restrictedAccess | |
dc.subject | Neonatal | |
dc.subject | Mice | |
dc.subject | DNA vaccine | |
dc.subject | HIV-1 | |
dc.subject | Immunological memory | |
dc.title | Immunization of neonatal mice with LAMP/p55 HIV gag DNA elicits robust immune responses that last to adulthood | |
dc.type | Artículos de revistas | |