dc.date.accessioned | 2017-04-27T18:52:36Z | |
dc.date.available | 2017-04-27T18:52:36Z | |
dc.date.created | 2017-04-27T18:52:36Z | |
dc.date.issued | 2009 | |
dc.identifier | 0014-2999 | |
dc.identifier | http://hdl.handle.net/10533/197715 | |
dc.identifier | D04I1240 | |
dc.identifier | WOS:000263016900035 | |
dc.identifier | WOS:000263016900035 | |
dc.description.abstract | The nuclear factor of activated T cells (NFAT) is a transcription factor essential for cytokine production during T-cell activation and is the target of several immunosuppressive drugs. Andrographolide is a diterpenic labdane that possesses anti-inflammatory and immunomodulatory effects. Several studies propose that andrographolide can reduce the immune response through inhibition of the nuclear factor kappa B (NF-kappaB) and mitogen-activated protein kinases (MAPK) such as extracellular signal regulated kinase 1/2 (ERK1/2) pathways. Moreover, andrographolide reduces IFN-gamma and IL-2 production induced by concanavalin A in murine T-cell. Nevertheless, the mechanisms involved in the decrease of cytokine production are unknown. In the present study, we determined that andrographolide reduced IL-2 production in Jurkat cells stimulated with phorbol myristate acetate and ionomycin (PMA/Ionomycin). We then showed that andrographolide reduced NFAT luciferase activity and interfered with its nuclear distribution, with these effects being linked to an increase in c-jun-N-terminal kinase (JNK) phosphorylation. Additionally, reduction of NF-kappaB activity in Jurkat cells treated with andrographolide was observed. Using Western blotting, we demonstrated that andrographolide decreased ERK1 and ERK5 phosphorylation induced by anti-CD3 or PMA/Ionomycin. Andrographolide did not affect cell viability at concentration of 10 and 50 muM; however, our results suggest that andrographolide increase early apoptosis at 100 muM. We concluded that andrographolide can exert immunomodulatory effects by interfering with NFAT activation and ERK1 and ERK5 phosphorylation in T-cells | |
dc.language | ENG | |
dc.publisher | ELSEVIER SCIENCE BV | |
dc.relation | https://doi.org/10.1016/j.ejphar.2008.11.011 | |
dc.relation | 10.1016/j.ejphar.2008.11.011 | |
dc.relation | info:eu-repo/grantAgreement/Fondef/D04I1240 | |
dc.relation | info:eu-repo/semantics/dataset/hdl.handle.net/10533/93477 | |
dc.relation | instname: Conicyt | |
dc.relation | reponame: Repositorio Digital RI2.0 | |
dc.relation | instname: Conicyt | |
dc.relation | reponame: Repositorio Digital RI2.0 | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.title | Andrographolide reduces il-2 production in t-cells by interfering with nfat and mapk activation | |
dc.type | Articulo | |