The aim of this paper is to explore a new analytical framework in the discussion of knowledge production and innovation processes. The main thesis is that the formation of networks is not contained in spatial configurations of a nation, region or localization. Therefore there is a need to explore new analytical perspectives in the era of globalization in order to comprehend their implications in the local space. Through a case study of nanotechnology knowledge networks in Northern Mexico, the formation of transnational knowledge spaces is analyzed by means of networks that connect plurilocal points and the circulation of knowledge. The analytical perspective proposed is a framework that discusses the knowledge production and innovation as an open space, which is socially produced, and in constant interaction between different actors and spatial scales. This paper aims to address the local space as an area of intersection, where multiple connections converge from different spatial scales: translocal, transregional, transnational.