Artículos de revistas
Solubility Of Lysozyme In Aqueous Solution Containing Ethanol Or Acetone: Unexpected Dependence On The Initial Protein Concentration
Registro en:
Fluid Phase Equilibria. Elsevier Science Bv, v. 429, p. 9 - 13, 2016.
0378-3812
1879-0224
WOS:000386414400002
10.1016/j.fluid.2016.08.024
Autor
Pinheiro
Marina Julio; Freitas
Sindelia; Miranda
Everson Alves; Pessoa Filho
Pedro de Alcantara
Institución
Resumen
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) This short communication presents unexpected experimental evidence that the solubility of lysozyme in aqueous solutions containing organic solvents (ethanol or acetone) depends not only on the fraction of the organic solvent, but also on the initial protein concentration (i.e., the concentration of protein before precipitation). The balance of enzymatic activity corroborates this fact. The observed shift in measured solubility is nearly proportional to the shift in initial protein concentration (up to 5-fold in ethanol solutions and up to 3-fold in acetone solutions). The dependence of solubility on the protein initial concentration has already been considered in literature as the formation of a second liquid-like phase instead of a solid precipitate, but calculated partition coefficients indicate that this description does not hold for these systems. While the results here presented do not allow an unequivocal description of the precipitate phase, they show that care must be exercised when describing protein precipitation with organic solvents. (C) 2016 Elsevier B.V. All rights reserved. 429 9 13 Brazilian funding agency FAPESP Brazilian funding agency CAPES Brazilian funding agency CPNq Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)