Artículos de revistas
Osteopontin Expression In Co-cultures Of Human Squamous Cell Carcinoma-derived Cells And Osteoblastic Cells And Its Effects On The Neoplastic Cell Phenotype And Osteoclastic Activation
Registro en:
Tumor Biology. Springer, v. 37, p. 12371 - 12385, 2016.
1010-4283
1423-0380
WOS:000387075400078
10.1007/s13277-016-5104-0
Autor
Teixeira
Lucas Novaes; Spinola de Castro Raucci
Larissa Moreira; Alonso
Gabriela Caroline; Della Coletta
Ricardo; Rosa
Adalberto Luiz; de Oliveira
Paulo Tambasco
Institución
Resumen
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) This study evaluated the temporal expression of osteopontin (OPN) in co-cultures of human osteoblastic cells (SAOS-2) and oral squamous cell carcinoma (OSCC)-derived cells (SCC9) and examined the effects of osteoblast-derived OPN on the neoplastic cell phenotype. Additionally, the effects of these co-cultures on subsequent osteoclastic activity were explored. SCC9 cells were plated on TranswellA (R) membranes that were either coated or not coated with Matrigel and were then co-cultured with SAOS-2 cells during the peak of OPN expression. SCC9 cells exposed to OPN-silenced SAOS-2 cultures and SCC9 cells cultured alone served as controls. SCC9 cells were quantitatively evaluated for cell adhesion, proliferation, migration, and invasion into Matrigel. The impact of co-culturing SAOS-2 and SCC9 cells on the resorptive capacity of U-937-derived osteoclastic cells was also investigated. Furthermore, a reciprocal induction of SAOS-2 and SCC9 cells in terms of OPN expression over the co-culture interval was identified. SAOS-2-secreted OPN altered the SCC9 cell phenotype, leading to enhanced cell adhesion and proliferation and higher Matrigel invasion. This invasion was also enhanced, albeit to a lesser degree, by co-culture with OPN-silenced SAOS-2 cells. Cell migration was not affected. Co-culture with SAOS-2 cells-mainly during the period of peak OPN expression-promoted over-expression of IL-6 and IL-8 by SCC9 cells and enhanced the resorptive capacity of osteoclastic cells. Taken together, these results suggest that osteoblast-derived OPN affects the interactions among OSCC-derived epithelial cells, osteoblasts, and osteoclasts, which could contribute to the process of bone destruction during bone invasion by OSCC. 37 9 12371 12385 State of Sao Paulo Research Foundation (FAPESP, Brazil) [2012/07531-9, 2012/20863-0, 2012/08605-6] National Council of Scientific and Technological Development (CNPq, Brazil) [308200/2012-8] Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)