Osteopontin Expression In Co-cultures Of Human Squamous Cell Carcinoma-derived Cells And Osteoblastic Cells And Its Effects On The Neoplastic Cell Phenotype And Osteoclastic Activation

dc.creatorTeixeira
dc.creatorLucas Novaes; Spinola de Castro Raucci
dc.creatorLarissa Moreira; Alonso
dc.creatorGabriela Caroline; Della Coletta
dc.creatorRicardo; Rosa
dc.creatorAdalberto Luiz; de Oliveira
dc.creatorPaulo Tambasco
dc.date2016
dc.dateset
dc.date2017-11-13T13:13:20Z
dc.date2017-11-13T13:13:20Z
dc.date.accessioned2018-03-29T05:51:18Z
dc.date.available2018-03-29T05:51:18Z
dc.identifierTumor Biology. Springer, v. 37, p. 12371 - 12385, 2016.
dc.identifier1010-4283
dc.identifier1423-0380
dc.identifierWOS:000387075400078
dc.identifier10.1007/s13277-016-5104-0
dc.identifierhttps://link.springer.com/article/10.1007%2Fs13277-016-5104-0
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/327029
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1364054
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionThis study evaluated the temporal expression of osteopontin (OPN) in co-cultures of human osteoblastic cells (SAOS-2) and oral squamous cell carcinoma (OSCC)-derived cells (SCC9) and examined the effects of osteoblast-derived OPN on the neoplastic cell phenotype. Additionally, the effects of these co-cultures on subsequent osteoclastic activity were explored. SCC9 cells were plated on TranswellA (R) membranes that were either coated or not coated with Matrigel and were then co-cultured with SAOS-2 cells during the peak of OPN expression. SCC9 cells exposed to OPN-silenced SAOS-2 cultures and SCC9 cells cultured alone served as controls. SCC9 cells were quantitatively evaluated for cell adhesion, proliferation, migration, and invasion into Matrigel. The impact of co-culturing SAOS-2 and SCC9 cells on the resorptive capacity of U-937-derived osteoclastic cells was also investigated. Furthermore, a reciprocal induction of SAOS-2 and SCC9 cells in terms of OPN expression over the co-culture interval was identified. SAOS-2-secreted OPN altered the SCC9 cell phenotype, leading to enhanced cell adhesion and proliferation and higher Matrigel invasion. This invasion was also enhanced, albeit to a lesser degree, by co-culture with OPN-silenced SAOS-2 cells. Cell migration was not affected. Co-culture with SAOS-2 cells-mainly during the period of peak OPN expression-promoted over-expression of IL-6 and IL-8 by SCC9 cells and enhanced the resorptive capacity of osteoclastic cells. Taken together, these results suggest that osteoblast-derived OPN affects the interactions among OSCC-derived epithelial cells, osteoblasts, and osteoclasts, which could contribute to the process of bone destruction during bone invasion by OSCC.
dc.description37
dc.description9
dc.description12371
dc.description12385
dc.descriptionState of Sao Paulo Research Foundation (FAPESP, Brazil) [2012/07531-9, 2012/20863-0, 2012/08605-6]
dc.descriptionNational Council of Scientific and Technological Development (CNPq, Brazil) [308200/2012-8]
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageEnglish
dc.publisherSpringer
dc.publisherDordrecht
dc.relationTumor Biology
dc.rightsfechado
dc.sourceWOS
dc.subjectOsteoblasts
dc.subjectOsteopontin
dc.subjectOral Squamous Cell Carcinoma
dc.subjectCo-culture
dc.subjectBone Invasion
dc.titleOsteopontin Expression In Co-cultures Of Human Squamous Cell Carcinoma-derived Cells And Osteoblastic Cells And Its Effects On The Neoplastic Cell Phenotype And Osteoclastic Activation
dc.typeArtículos de revistas


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