Artículos de revistas
Functional And Genetic Characterization Of Hydrocarbon Biodegrader And Exopolymer-producing Clones From A Petroleum Reservoir Metagenomic Library
Registro en:
Environmental Technology . Taylor & Francis Ltd, v. 38, p. 1139 - 1150, 2017.
0959-3330
1479-487X
WOS:000397972100009
10.1080/09593330.2016.1218940
Autor
Vasconcellos
Suzan P.; Sierra-Garcia
Isabel N.; Dellagnezze
Bruna M.; Vicentini
Renato; Midgley
David; Silva
Cynthia C.; Neto
Eugenio V. Santos; Volk
Herbert; Hendry
Philip; Oliveira
Valeria M.
Institución
Resumen
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Microbial degradation of petroleum is a worldwide issue, which causes physico-chemical changes in its compounds, diminishing its commercial value. Biosurfactants are chemically diverse molecules that can be produced by several microorganisms and can enable microbial access to hydrocarbons. In order to investigate both microbial activities, function-driven screening assays for biosurfactant production and hydrocarbon biodegradation were carried out from a metagenomic fosmid library. It was constructed from the total DNA extracted from aerobic and anaerobic enrichments from a Brazilian biodegraded petroleum sample. A sum of 10 clones were selected in order to evaluate their ability to produce exopolymers (EPS) with emulsifying activity, as well as to characterize the gene sequences, harbored by the fosmid clones, through 454 pyrosequencing. Functional analyses confirmed the ability of some clones to produce surfactant compounds. Regarding hydrocarbon as microbial carbon sources, n-alkane (in mixture or not) and naphthalene were preferentially consumed as substrates. Analysis of sequence data set revealed the presence of genes related to xenobiotics biodegradation and carbohydrate metabolism. These data were corroborated by the results of hydrocarbon biodegradation and biosurfactant production detected in the evaluated clones. 38 9 1139 1150 FundaCao de Amparo a Pesquisa do Estado de Sao Paulo [2006/57401-3] Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)