Functional And Genetic Characterization Of Hydrocarbon Biodegrader And Exopolymer-producing Clones From A Petroleum Reservoir Metagenomic Library

dc.creatorVasconcellos
dc.creatorSuzan P.; Sierra-Garcia
dc.creatorIsabel N.; Dellagnezze
dc.creatorBruna M.; Vicentini
dc.creatorRenato; Midgley
dc.creatorDavid; Silva
dc.creatorCynthia C.; Neto
dc.creatorEugenio V. Santos; Volk
dc.creatorHerbert; Hendry
dc.creatorPhilip; Oliveira
dc.creatorValeria M.
dc.date2017
dc.date2017-11-13T13:12:23Z
dc.date2017-11-13T13:12:23Z
dc.date.accessioned2018-03-29T05:50:36Z
dc.date.available2018-03-29T05:50:36Z
dc.identifierEnvironmental Technology . Taylor & Francis Ltd, v. 38, p. 1139 - 1150, 2017.
dc.identifier0959-3330
dc.identifier1479-487X
dc.identifierWOS:000397972100009
dc.identifier10.1080/09593330.2016.1218940
dc.identifierhttp://www.tandfonline.com/doi/full/10.1080/09593330.2016.1218940
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/326862
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1363887
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionMicrobial degradation of petroleum is a worldwide issue, which causes physico-chemical changes in its compounds, diminishing its commercial value. Biosurfactants are chemically diverse molecules that can be produced by several microorganisms and can enable microbial access to hydrocarbons. In order to investigate both microbial activities, function-driven screening assays for biosurfactant production and hydrocarbon biodegradation were carried out from a metagenomic fosmid library. It was constructed from the total DNA extracted from aerobic and anaerobic enrichments from a Brazilian biodegraded petroleum sample. A sum of 10 clones were selected in order to evaluate their ability to produce exopolymers (EPS) with emulsifying activity, as well as to characterize the gene sequences, harbored by the fosmid clones, through 454 pyrosequencing. Functional analyses confirmed the ability of some clones to produce surfactant compounds. Regarding hydrocarbon as microbial carbon sources, n-alkane (in mixture or not) and naphthalene were preferentially consumed as substrates. Analysis of sequence data set revealed the presence of genes related to xenobiotics biodegradation and carbohydrate metabolism. These data were corroborated by the results of hydrocarbon biodegradation and biosurfactant production detected in the evaluated clones.
dc.description38
dc.description9
dc.description1139
dc.description1150
dc.descriptionFundaCao de Amparo a Pesquisa do Estado de Sao Paulo [2006/57401-3]
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageEnglish
dc.publisherTaylor & Francis Ltd
dc.publisherAbingdon
dc.relationEnvironmental Technology
dc.rightsfechado
dc.sourceWOS
dc.subjectFosmid Clones
dc.subjectHeterologous Expression
dc.subjectBiosurfactant
dc.subjectHydrocarbon
dc.subjectBiodegradation
dc.titleFunctional And Genetic Characterization Of Hydrocarbon Biodegrader And Exopolymer-producing Clones From A Petroleum Reservoir Metagenomic Library
dc.typeArtículos de revistas


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