Artículos de revistas
Dietary Interesterified Fat Enriched With Palmitic Acid Induces Atherosclerosis By Impairing Macrophage Cholesterol Efflux And Eliciting Inflammation
Registro en:
The Journal Of Nutritional Biochemistry. Elsevier Science Inc, v. 32, p. 91 - 100, 2016.
0955-2863
1873-4847
WOS:000377634300010
10.1016/j.jnutbio.2016.01.005
Autor
Afonso
Milessa Silva; Ferrari Lavrador
Maria Silvia; Koike
Marcia Kiyomi; Cintra
Dennys Esper; Ferreira
Fabiana Dias; Nunes
Valeria Sutti; Castilho
Gabriela; Gioielli
Luiz Antonio; Bombo
Renata Paula; Catanozi
Sergio; Caldini
Elia Garcia; Damaceno-Rodrigues
Nilsa Regina; Passarelli
Marisa; Nakandakare
Edna Regina; Lottenberg
Ana Maria
Institución
Resumen
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Interesterified fats are currently being used to replace trans fatty acids. However, their impact on biological pathways involved in the atherosclerosis development was not investigated. Weaning male LDLr-KO mice were fed for 16 weeks on a high-fat diet (40% energy as fat) containing polyunsaturated (PUFA), TRANS, palmitic (PALM), palmitic interesterified (PALM INTER), stearic (STEAR) or stearic interesterified (STEAR INTER). Plasma lipids, lipoprotein profile, arterial lesion area, macrophage infiltration, collagen content and inflammatory response modulation were determined. Macrophage cholesterol efflux and the arterial expression of cholesterol uptake and efflux receptors were also performed. The interesterification process did not alter plasma lipid concentrations. Although PALM INTER did not increase plasma cholesterol concentration as much as TRANS, the cholesterol enrichment in the LDL particle was similar in both groups. Moreover, PALM INTER induced the highest IL-1 beta, MCP-1 and IL-6 secretion from peritoneal macrophages as compared to others. This inflammatory response elicited by PALM INTER was confirmed in arterial wall, as compared to PALM. These deleterious effects of PALM INTER culminate in higher atherosclerotic lesion, macrophage infiltration and collagen content than PALM, STEAR, STEAR INTER and PUFA. These events can partially be attributed to a macrophage cholesterol accumulation, promoted by apoAl and HDL2-mediated cholesterol efflux impairment and increased Olr-1 and decreased Abca1 and Nr1h3 expressions in the arterial wall. Interesterified fats containing palmitic acid induce atherosclerosis development by promoting cholesterol accumulation in LDL particles and macrophagic cells, activating the inflammatory process in LDLr-KO mice. (C) 2016 Elsevier Inc. All rights reserved. 32 91 100 State of Sao Paulo Research Foundation (FAPESP) [11/50443-0, 12/50249-2] Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)