Comparative Proteomics And Metallomics Studies In Arabidopsis Thaliana Leaf Tissues: Evaluation Of The Selenium Addition In Transgenic And Nontransgenic Plants Using Two-dimensional Difference Gel Electrophoresis And Laser Ablation Imaging.

dc.creatorMaciel, Bruna C M
dc.creatorBarbosa, Herbert S
dc.creatorPessôa, Gustavo S
dc.creatorSalazar, Marcela M
dc.creatorPereira, Gonçalo A G
dc.creatorGonçalves, Danieli C
dc.creatorRamos, Carlos H I
dc.creatorArruda, Marco A Z
dc.date2014-Apr
dc.date2015-11-27T13:42:13Z
dc.date2015-11-27T13:42:13Z
dc.date.accessioned2018-03-29T01:20:16Z
dc.date.available2018-03-29T01:20:16Z
dc.identifierProteomics. v. 14, n. 7-8, p. 904-12, 2014-Apr.
dc.identifier1615-9861
dc.identifier10.1002/pmic.201300427
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/24678036
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/201270
dc.identifier24678036
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1301503
dc.descriptionThe main goal of this work is to evaluate some differential protein species in transgenic (T) and nontransgenic (NT) Arabidopsis thaliana plants after their cultivation in the presence or absence of sodium selenite. The transgenic line was obtained through insertion of CaMV 35S controlling nptII gene. Comparative proteomics through 2D-DIGE is carried out in four different groups (NT × T; NT × Se-NT (where Se is selenium); Se-NT × Se-T, and T × Se-T). Although no differential proteins are achieved in the T × Se-T group, for the others, 68 differential proteins (by applying a regulation factor ≥1.5) are achieved, and 27 of them accurately characterized by ESI-MS/MS. These proteins are classified into metabolism, energy, signal transduction, disease/defense categories, and some of them are involved in the glycolysis pathway-Photosystems I and II and ROS combat. Additionally, laser ablation imaging is used for evaluating the Se and sulfur distribution in leaves of different groups, corroborating some results obtained and related to proteins involved in the glycolysis pathway. From these results, it is possible to conclude that the genetic modification also confers to the plant resistance to oxidative stress.
dc.description14
dc.description904-12
dc.languageeng
dc.relationProteomics
dc.relationProteomics
dc.rightsfechado
dc.rights© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
dc.sourcePubMed
dc.subjectArabidopsis
dc.subjectElectrophoresis, Gel, Two-dimensional
dc.subjectGene Expression Regulation, Plant
dc.subjectLasers
dc.subjectMolecular Imaging
dc.subjectPlant Leaves
dc.subjectPlant Proteins
dc.subjectPlants, Genetically Modified
dc.subjectProteomics
dc.subjectSodium Selenite
dc.subjectMetallomics
dc.subjectOxidative Stress
dc.subjectPlant Proteomics
dc.subjectSelenium
dc.subjectTransgenic Plant
dc.titleComparative Proteomics And Metallomics Studies In Arabidopsis Thaliana Leaf Tissues: Evaluation Of The Selenium Addition In Transgenic And Nontransgenic Plants Using Two-dimensional Difference Gel Electrophoresis And Laser Ablation Imaging.
dc.typeArtículos de revistas


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