P2x3 Receptors Induced Inflammatory Nociception Modulated By Trpa1, 5-ht3 And 5-ht1a Receptors.

dc.creatorKrimon, Suzy
dc.creatorAraldi, Dionéia
dc.creatordo Prado, Filipe César
dc.creatorTambeli, Cláudia Herrera
dc.creatorOliveira-Fusaro, Maria Cláudia G
dc.creatorParada, Carlos Amílcar
dc.date2013-Nov
dc.date2015-11-27T13:32:13Z
dc.date2015-11-27T13:32:13Z
dc.date.accessioned2018-03-29T01:18:37Z
dc.date.available2018-03-29T01:18:37Z
dc.identifierPharmacology, Biochemistry, And Behavior. v. 112, p. 49-55, 2013-Nov.
dc.identifier1873-5177
dc.identifier10.1016/j.pbb.2013.09.017
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/24120766
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/200844
dc.identifier24120766
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1301077
dc.descriptionIt has been described that endogenous ATP via activation of P2X3 and P2X2/3 receptors contributes to inflammatory nociception in different models, including the formalin injected in subcutaneous tissue of the rat's hind paw. In this study, we have evaluated whether TRPA1, 5-HT3 and 5-HT1A receptors, whose activation is essential to formalin-induced inflammatory nociception, are involved in the nociception induced by activation of P2X3 receptors on subcutaneous tissue of the rat's hind paw. We have also evaluated whether the activation of P2X3 receptors increases the susceptibility of primary afferent neurons to formalin action modulated by activation of TRPA1, 5-HT3 or 5-HT1A receptors. Nociceptive response intensity was measured by observing the rat's behavior and considering the number of times the animal reflexively raised its hind paw (flinches) in 60min. Local subcutaneous administration of the selective TRPA1, 5-HT3 or 5-HT1A receptor antagonists HC 030031, tropisetron and WAY 100,135, respectively, prevented the nociceptive responses induced by the administration in the same site of the non-selective P2X3 receptor agonist αβmeATP. Administration of the selective P2X3 and P2X2/3 receptor antagonist A-317491 or pretreatment with oligonucleotides antisense against P2X3 receptor prevented the formalin-induced behavioral nociceptive responses during the first and second phases. Also, the co-administration of a subthreshold dose of αβmeATP with a subthreshold dose of formalin induced nociceptive behavior, which was prevented by local administration of tropisetron, HC 030031 or WAY 100, 135. These findings have demonstrated that the activation of P2X3 receptors induces inflammatory nociception modulated by TRPA1, 5-HT3 and 5-HT1A receptors. Also, they suggest that inflammatory nociception is modulated by the release of endogenous ATP and P2X3 receptor activation, which in turn, increases primary afferent nociceptor susceptibility to the action of inflammatory mediators via interaction with TRPA1, 5-HT3 and 5-HT1A receptors in the peripheral tissue.
dc.description112
dc.description49-55
dc.languageeng
dc.relationPharmacology, Biochemistry, And Behavior
dc.relationPharmacol. Biochem. Behav.
dc.rightsfechado
dc.rights© 2013.
dc.sourcePubMed
dc.subjectAnimals
dc.subjectBlotting, Western
dc.subjectMale
dc.subjectNociception
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectReceptor, Serotonin, 5-ht1a
dc.subjectReceptors, Purinergic P2x3
dc.subjectReceptors, Serotonin, 5-ht3
dc.subjectTrpc Cation Channels
dc.subject5-ht3 Receptors
dc.subjectFormalin
dc.subjectP2x3 Receptors
dc.subjectTrpa1 Receptors
dc.titleP2x3 Receptors Induced Inflammatory Nociception Modulated By Trpa1, 5-ht3 And 5-ht1a Receptors.
dc.typeArtículos de revistas


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