Artículos de revistas
Antiproliferative Effect Of Benzophenones And Their Influence On Cathepsin Activity.
Registration in:
Phytotherapy Research : Ptr. v. 24, n. 3, p. 379-83, 2010-Mar.
1099-1573
10.1002/ptr.2954
19653314
Author
Murata, Ramiro Mendonça
Yatsuda, Regiane
dos Santos, Marcelo Henrique
Kohn, Luciana K
Martins, Felipe Terra
Nagem, Tanus Jorge
Alencar, Severino Matias
de Carvalho, João Ernesto
Rosalen, Pedro Luiz
Institutions
Abstract
The antiproliferative activity of two prenylated benzophenones isolated from Rheedia brasiliensis, the triprenylated garciniaphenone and the tetraprenylated benzophenone 7-epiclusianone, was investigated against human cancer cell lines. The antiproliferative activity on melanoma (UACC-62), breast (MCF-7), drug-resistant breast (NCI-ADR), lung/non-small cells (NCI460), ovarian (OVCAR 03), prostate (PC03), kidney (786-0), lung (NCI-460) and tongue (CRL-1624 and CRL-1623) cancer cells was determined using spectrophotometric quantification of the cellular protein content. The effect of these benzophenones on the activity of cathepsins B and G was also investigated. Garciniaphenone displayed cytostatic activity in all cell lines, whereas 7-epiclusianone showed a dose-dependent cytotoxic effect. The IC(50) values for cell proliferation revealed that 7-epiclusianone is more active than garciniaphenone against most of the cell lines. Furthermore, the antiproliferative effects demonstrated by garciniaphenone and 7-epiclusianone were related to their cathepsin inhibiting properties. In conclusion, 7-epiclusianone is a promising naturally occurring agent which displays multiple inhibitory effects which may be working in concert to inhibit cancer cell proliferation in vitro. The putative pathway by which 7-epiclusianone affects cancer cell development may involve cathepsin inhibition. 24 379-83