Characterization Of A Sugarcane (saccharum Spp.) Gene Homolog To The Brassinosteroid Insensitive1-associated Receptor Kinase 1 That Is Associated To Sugar Content.

dc.creatorVicentini, Renato
dc.creatorFelix, Juliana de Maria
dc.creatorDornelas, Marcelo Carnier
dc.creatorMenossi, Marcelo
dc.date2009-Mar
dc.date2015-11-27T13:15:53Z
dc.date2015-11-27T13:15:53Z
dc.date.accessioned2018-03-29T01:10:03Z
dc.date.available2018-03-29T01:10:03Z
dc.identifierPlant Cell Reports. v. 28, n. 3, p. 481-91, 2009-Mar.
dc.identifier1432-203X
dc.identifier10.1007/s00299-008-0656-0
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/19096852
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/198631
dc.identifier19096852
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1298864
dc.descriptionThe present article reports on the characterization of ScBAK1, a leucine-rich repeat receptor-like kinase from sugarcane (Saccharum spp.), expressed predominantly in bundle-sheath cells of the mature leaf and potentially involved in cellular signaling cascades mediated by high levels of sugar in this organ. In this report, it was shown that the ScBAK1 sequence was similar to the brassinosteroid insensitive1-associated receptor kinase1 (BAK1). The putative cytoplasmatic domain of ScBAK1 contains all the amino acids characteristic of protein kinases, and the extracellular domain contains five leucine-rich repeats and a putative leucine zipper. Transcripts of ScBAK1 were almost undetectable in sugarcane roots or in any other sink tissue, but accumulated abundantly in the mature leaves. The ScBAK1 expression was higher in the higher sugar content individuals from a population segregating for sugar content throughout the growing season. In situ hybridization in sugarcane leaves showed that the ScBAK1 mRNA accumulated at much higher levels in bundle-sheath cells than in mesophyll cells. In addition, using biolistic bombardment of onion epidermal cells, it was shown that ScBAK1-GFP fusions were localized in the plasma membrane as predicted for a receptor kinase. All together, the present data indicate that ScBAK1 might be a receptor involved in the regulation of specific processes in bundle-sheath cells and in sucrose synthesis in mature sugarcane leaves.
dc.description28
dc.description481-91
dc.languageeng
dc.relationPlant Cell Reports
dc.relationPlant Cell Rep.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAmino Acid Sequence
dc.subjectCell Membrane
dc.subjectGene Expression Profiling
dc.subjectGene Expression Regulation, Plant
dc.subjectGenes, Plant
dc.subjectMolecular Sequence Data
dc.subjectOligonucleotide Array Sequence Analysis
dc.subjectPhylogeny
dc.subjectPlant Leaves
dc.subjectPlant Proteins
dc.subjectPlants, Genetically Modified
dc.subjectProtein-serine-threonine Kinases
dc.subjectRna, Messenger
dc.subjectRna, Plant
dc.subjectSaccharum
dc.subjectSucrose
dc.titleCharacterization Of A Sugarcane (saccharum Spp.) Gene Homolog To The Brassinosteroid Insensitive1-associated Receptor Kinase 1 That Is Associated To Sugar Content.
dc.typeArtículos de revistas


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