Peripheral Mechanisms Underlying The Essential Role Of P2x3,2/3 Receptors In The Development Of Inflammatory Hyperalgesia.

dc.creatorOliveira, Maria Cláudia G
dc.creatorPelegrini-da-Silva, Adriana
dc.creatorTambeli, Cláudia Herrera
dc.creatorParada, Carlos Amílcar
dc.date2009-Jan
dc.date2015-11-27T13:15:52Z
dc.date2015-11-27T13:15:52Z
dc.date.accessioned2018-03-29T01:10:02Z
dc.date.available2018-03-29T01:10:02Z
dc.identifierPain. v. 141, n. 1-2, p. 127-34, 2009-Jan.
dc.identifier1872-6623
dc.identifier10.1016/j.pain.2008.10.024
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/19081189
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/198626
dc.identifier19081189
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1298859
dc.descriptionActivation of P2X3,2/3 receptors by endogenous ATP contributes to the development of inflammatory hyperalgesia. Given the clinical importance of mechanical hyperalgesia in inflammatory states, we hypothesized that the activation of P2X3,2/3 receptors by endogenous ATP contributes to carrageenan-induced mechanical hyperalgesia and that this contribution is mediated by an indirect and/or a direct sensitization of the primary afferent nociceptors. Co-administration of the selective P2X3,2/3 receptors antagonist A-317491, or the non-selective P2X3 receptor antagonist, TNP-ATP, with carrageenan blocked the mechanical hyperalgesia induced by carrageenan, and significantly reduced the increased concentration of tumor necrosis factor alpha (TNF-alpha) and chemokine-induced chemoattractant-1 (CINC-1) but not of interleukin-1 beta (IL-1 beta) induced by carrageenan. Co-administration of the selective P2X3,2/3 receptors antagonist A-317491 with carrageenan did not affect the neutrophil migration induced by carrageenan. Intrathecal administration of oligonucleotides antisense against P2X3 receptors for seven days significantly reduced the expression of P2X3 receptors in the saphenous nerve and significantly reduced the mechanical hyperalgesia induced by carrageenan. We concluded that the activation of P2X3,2/3 receptors by endogenous ATP is essential to the development of the mechanical hyperalgesia induced by carrageenan. Furthermore, we showed that this essential role of P2X3,2/3 receptors in the development of carrageenan-induced mechanical hyperalgesia is mediated by an indirect sensitization of the primary afferent nociceptors dependent on the previous release of TNF-alpha and by a direct sensitization of the primary afferent nociceptors.
dc.description141
dc.description127-34
dc.languageeng
dc.relationPain
dc.relationPain
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAdenosine Triphosphate
dc.subjectAnalysis Of Variance
dc.subjectAnimals
dc.subjectCarrageenan
dc.subjectCytokines
dc.subjectDisease Models, Animal
dc.subjectDose-response Relationship, Drug
dc.subjectDrug Administration Routes
dc.subjectEnzyme-linked Immunosorbent Assay
dc.subjectHyperalgesia
dc.subjectInflammation
dc.subjectMale
dc.subjectOligodeoxyribonucleotides, Antisense
dc.subjectPain Measurement
dc.subjectPain Threshold
dc.subjectPeroxidase
dc.subjectPhenols
dc.subjectPolycyclic Compounds
dc.subjectPolysaccharides
dc.subjectPurinergic P2 Receptor Antagonists
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectReceptors, Purinergic P2
dc.subjectReceptors, Purinergic P2x2
dc.subjectReceptors, Purinergic P2x3
dc.subjectTime Factors
dc.titlePeripheral Mechanisms Underlying The Essential Role Of P2x3,2/3 Receptors In The Development Of Inflammatory Hyperalgesia.
dc.typeArtículos de revistas


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