Artículos de revistas
Clonazepam Quantification In Human Plasma By High-performance Liquid Chromatography Coupled With Electrospray Tandem Mass Spectrometry In A Bioequivalence Study.
Registro en:
Journal Of Mass Spectrometry : Jms. v. 42, n. 1, p. 81-8, 2007-Jan.
1076-5174
10.1002/jms.1141
17154437
Autor
Cavedal, Luiz E
Mendes, Fabiana D
Domingues, Claudia C
Patni, Anil K
Monif, Tausif
Reyar, Simrit
Pereira, Alberto Dos S
Mendes, Gustavo D
De Nucci, Gilberto
Institución
Resumen
A rapid, sensitive and specific method for quantifying clonazepam in human plasma using diazepam as the internal standard (IS) is described. The analyte and the IS were extracted from plasma by liquid-liquid extraction using a hexane/diethylether (20 : 80, v/v) solution. The extracts were analysed by high-performance liquid chromatography coupled with electrospray tandem mass spectrometry (HPLC-MS-MS). Chromatography was performed on a Jones Genesis C8 4 microm analytical column (100 x 2.1 mm i.d.). The method had a chromatographic run time of 3.0 min and a linear calibration curve over the range 0.5-50 ng/ml (r2 > 0.9965). The limit of quantification was 0.5 ng/ml. This HPLC/MS/MS procedure was used to assess the bioequivalence of two clonazepam 2 mg tablet formulations (clonazepam test formulation from Ranbaxy Laboratories Ltd and Rivotril from Roche Laboratórios Ltda as standard reference formulation). 42 81-8