Brasil | Artículos de revistas

Clonazepam Quantification In Human Plasma By High-performance Liquid Chromatography Coupled With Electrospray Tandem Mass Spectrometry In A Bioequivalence Study.

dc.creatorCavedal, Luiz E
dc.creatorMendes, Fabiana D
dc.creatorDomingues, Claudia C
dc.creatorPatni, Anil K
dc.creatorMonif, Tausif
dc.creatorReyar, Simrit
dc.creatorPereira, Alberto Dos S
dc.creatorMendes, Gustavo D
dc.creatorDe Nucci, Gilberto
dc.date2007-Jan
dc.date2015-11-27T13:10:35Z
dc.date2015-11-27T13:10:35Z
dc.date.accessioned2018-03-29T01:05:40Z
dc.date.available2018-03-29T01:05:40Z
dc.identifierJournal Of Mass Spectrometry : Jms. v. 42, n. 1, p. 81-8, 2007-Jan.
dc.identifier1076-5174
dc.identifier10.1002/jms.1141
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/17154437
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/197514
dc.identifier17154437
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1297747
dc.descriptionA rapid, sensitive and specific method for quantifying clonazepam in human plasma using diazepam as the internal standard (IS) is described. The analyte and the IS were extracted from plasma by liquid-liquid extraction using a hexane/diethylether (20 : 80, v/v) solution. The extracts were analysed by high-performance liquid chromatography coupled with electrospray tandem mass spectrometry (HPLC-MS-MS). Chromatography was performed on a Jones Genesis C8 4 microm analytical column (100 x 2.1 mm i.d.). The method had a chromatographic run time of 3.0 min and a linear calibration curve over the range 0.5-50 ng/ml (r2 > 0.9965). The limit of quantification was 0.5 ng/ml. This HPLC/MS/MS procedure was used to assess the bioequivalence of two clonazepam 2 mg tablet formulations (clonazepam test formulation from Ranbaxy Laboratories Ltd and Rivotril from Roche Laboratórios Ltda as standard reference formulation).
dc.description42
dc.description81-8
dc.languageeng
dc.relationJournal Of Mass Spectrometry : Jms
dc.relationJ Mass Spectrom
dc.rightsfechado
dc.rightsCopyright 2006 John Wiley & Sons, Ltd.
dc.sourcePubMed
dc.subjectAdolescent
dc.subjectAdult
dc.subjectAnticonvulsants
dc.subjectChromatography, High Pressure Liquid
dc.subjectClonazepam
dc.subjectCross-over Studies
dc.subjectDioxanes
dc.subjectHexanes
dc.subjectHumans
dc.subjectMiddle Aged
dc.subjectSpectrometry, Mass, Electrospray Ionization
dc.subjectTandem Mass Spectrometry
dc.subjectTherapeutic Equivalency
dc.titleClonazepam Quantification In Human Plasma By High-performance Liquid Chromatography Coupled With Electrospray Tandem Mass Spectrometry In A Bioequivalence Study.
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución