Defects in insulin signal transduction in liver and muscle of pregnant rats

dc.creatorSaad, MJA
dc.creatorMaeda, L
dc.creatorBrenelli, SL
dc.creatorCarvalho, CRO
dc.creatorPaiva, RS
dc.creatorVelloso, LA
dc.date1997
dc.dateFEB
dc.date2014-12-16T11:36:43Z
dc.date2015-11-26T17:26:23Z
dc.date2014-12-16T11:36:43Z
dc.date2015-11-26T17:26:23Z
dc.date.accessioned2018-03-29T00:13:35Z
dc.date.available2018-03-29T00:13:35Z
dc.identifierDiabetologia. Springer Verlag, v. 40, n. 2, n. 179, n. 186, 1997.
dc.identifier0012-186X
dc.identifierWOS:A1997WJ10600008
dc.identifier10.1007/s001250050660
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/79374
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/79374
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/79374
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1284549
dc.descriptionPregnancy is known to induce insulin resistance, but the exact molecular mechanism involved is unknown. In the present study, we have examined the levels and phosphorylation state of the insulin receptor and of insulin receptor substrate 1(IRS-1), as well as the association between IRS-1 and phosphatidylinositol 3-kinase (PI 3-kinase) in the liver and muscle of pregnant rats (day 20 of gestation) by immunoprecipitation and immunoblotting with anti-insulin receptor, anti-IRS-1, anti-PI 3-kinase and antiphosphotyrosine antibodies. There were no changes in the insulin receptor concentration in the liver and muscle of pregnant rats. However, insulin stimulation of receptor autophosphorylation, as determined by immunoblotting with antiphosphotyrosine antibody, was reduced by 30+/-6% (p < 0.02) in muscle and 36+/-5% (p < 0.01) in liver at day 20 of gestation. IRS-1 protein levels decreased by 45+/-6% (p < 0.002) in liver and by 56+/-9% (p < 0.002) in muscle of pregnant rats. In samples previously immunoprecipitated with anti-IRS-1 antibody and blotted with antiphosphotyrosine antibody, the insulin-stimulated IRS-1 phosphorylation levels in the muscle and liver of pregnant rats decreased by 70+/-9% (p < 0.01) and 75+/-8% (p < 0.01), respectively. The insulin-stimulated IRS-1 association with PI 3-kinase decreased by 81+/-6% in muscle (p < 0.01) and 79+/-11% (p < 0.01) in the liver during pregnancy. These data suggest that changes in the early steps of insulin signal transduction may have a role in the insulin resistance observed in pregnancy.
dc.description40
dc.description2
dc.description179
dc.description186
dc.languageen
dc.publisherSpringer Verlag
dc.publisherNew York
dc.relationDiabetologia
dc.relationDiabetologia
dc.rightsfechado
dc.rightshttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.sourceWeb of Science
dc.subjectinsulin resistance
dc.subjectpregnancy
dc.subjectinsulin action
dc.subjectinsulin receptor
dc.subjectinsulin receptor substrate
dc.subjectPI 3-kinase
dc.subjectActivated Protein-kinase
dc.subjectPhosphatidylinositol 3-kinase Activity
dc.subjectGlycogen-synthase Kinase-3
dc.subjectReceptor Substrate-1
dc.subjectSkeletal-muscle
dc.subjectTyrosine Kinase
dc.subjectGlut4 Translocation
dc.subjectGlucose-transporter
dc.subjectClamp Technique
dc.subjectRas Activation
dc.titleDefects in insulin signal transduction in liver and muscle of pregnant rats
dc.typeArtículos de revistas


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