Artículos de revistas
Dendritic cells treated with chloroquine modulate experimental autoimmune encephalomyelitis
Registro en:
Immunology And Cell Biology. Nature Publishing Group, v. 92, n. 2, n. 124, n. 132, 2014.
0818-9641
1440-1711
WOS:000330999500006
10.1038/icb.2013.73
Autor
Thome, R
Issayama, LK
DiGangi, R
Bombeiro, AL
da Costa, TA
Ferreira, IT
de Oliveira, ALR
Verinaud, L
Institución
Resumen
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Chloroquine (CQ), an antimalarial drug, has been shown to modulate the immune system and reduce the severity of experimental autoimmune encephalomyelitis (EAE). The mechanisms of disease suppression are dependent on regulatory T cell induction, although Tregs-independent mechanisms exist. We aimed to evaluate whether CQ is capable to modulate bone marrow-derived dendritic cells (DCs) both phenotypically and functionally as well as whether transfer of CQ-modulated DCs reduces EAE course. Our results show that CQ-treated DCs presented altered ultrastructure morphology and lower expression of molecules involved in antigen presentation. Consequently, T cell proliferation was diminished in coculture experiments. When transferred into EAE mice, DC-CQ was able to reduce the clinical manifestation of the disease through the modulation of the immune response against neuroantigens. The data presented herein indicate that chloroquine-mediated modulation of the immune system is achieved by a direct effect on DCs and that DC-CQ adoptive transfer may be a promising approach for avoiding drug toxicity. 92 2 124 132 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) FAPESP [2011/17965-3] FAPESP [2011/13191-3, 2012/08303-0]