The variation of total protein of spore during germination of strains E9, Pegro and BSA of Metarhizium anisopliae var. anisopliae was investigated. Minimal germination time (MGT, defined as the time during which 85% of the conidia germinated) for the three strains was 12 hours of incubation in minimal medium. When the total protein content of the three strains was determined, variations related to increased germination time were detected and the beginning of protein synthesis was found to differ among strains. Strain E9 started protein synthesis between 0 and 7 hours of germination time, whereas strains Pegro and BSA started germination between 7 and 13 hours.274268272