Proteome analysis of the plasma protein layer adsorbed to a rough titanium surface

dc.creatorDodo, CG
dc.creatorSenna, PM
dc.creatorCustodio, W
dc.creatorLeme, AFP
dc.creatorCury, AAD
dc.date2013
dc.dateMAY 1
dc.date2014-07-30T14:18:28Z
dc.date2015-11-26T16:41:04Z
dc.date2014-07-30T14:18:28Z
dc.date2015-11-26T16:41:04Z
dc.date.accessioned2018-03-28T23:25:10Z
dc.date.available2018-03-28T23:25:10Z
dc.identifierBiofouling. Taylor & Francis Ltd, v. 29, n. 5, n. 549, n. 557, 2013.
dc.identifier0892-7014
dc.identifierWOS:000319740800007
dc.identifier10.1080/08927014.2013.787416
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/58343
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/58343
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1272806
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionIn this study a label-free proteomic approach was used to investigate the composition of the layer of protein adsorbed to rough titanium (Ti) after exposure to human blood plasma. The influence of the protein layer on the surface free energy (SFE) of the Ti was evaluated by contact angle measurements. Ti discs were incubated with blood plasma for 180min at 37 degrees C, and the proteins recovered were subjected to liquid chromatography coupled to tandem mass spectrometry analysis. A total of 129 different peptides were identified and assigned to 25 distinct plasma proteins. The most abundant proteins were fibronectin, serum albumin, apolipoprotein A-I, and fibrinogen, comprising 74.54% of the total spectral counts. Moreover, the protein layer increased the SFE of the Ti (p<0.05). The layer adsorbed to the rough Ti surface was composed mainly of proteins related to cell adhesion, molecule transportation, and coagulation processes, creating a polar and hydrophilic interface for subsequent interactions with host cells.
dc.description29
dc.description5
dc.description549
dc.description557
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionBrasilia, DF, Brazil) [471553/2010-7]
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFAPESP [2010/09308-0, 2010/09113-4]
dc.descriptionBrasilia, DF, Brazil) [471553/2010-7]
dc.languageen
dc.publisherTaylor & Francis Ltd
dc.publisherAbingdon
dc.publisherInglaterra
dc.relationBiofouling
dc.relationBiofouling
dc.rightsfechado
dc.rightshttp://journalauthors.tandf.co.uk/permissions/reusingOwnWork.asp
dc.sourceWeb of Science
dc.subjecttitanium
dc.subjectsurface properties
dc.subjectsurface-free energy
dc.subjectprotein adsorption
dc.subjecthuman blood plasma
dc.subjectproteomics
dc.subjectAcquired Enamel Pellicle
dc.subjectMass-spectrometry
dc.subjectSolid-surfaces
dc.subjectOsteoblast Adhesion
dc.subjectAlbumin Adsorption
dc.subjectImplant Surfaces
dc.subjectCell-adhesion
dc.subjectPilot Phase
dc.subjectFibronectin
dc.subjectPeptide
dc.titleProteome analysis of the plasma protein layer adsorbed to a rough titanium surface
dc.typeArtículos de revistas


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