Characterization of a mixed-mode reversed-phase/cation-exchange stationary phase prepared by thermal immobilization of poly(dimethylsiloxane) onto the surface of silica

dc.creatorBorges, EM
dc.creatorEuerby, MR
dc.creatorCollins, CH
dc.date2012
dc.dateFEB
dc.date2014-07-30T16:51:51Z
dc.date2015-11-26T16:35:26Z
dc.date2014-07-30T16:51:51Z
dc.date2015-11-26T16:35:26Z
dc.date.accessioned2018-03-28T23:17:54Z
dc.date.available2018-03-28T23:17:54Z
dc.identifierAnalytical And Bioanalytical Chemistry. Springer Heidelberg, v. 402, n. 6, n. 2043, n. 2055, 2012.
dc.identifier1618-2642
dc.identifierWOS:000300308400007
dc.identifier10.1007/s00216-011-5674-z
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/62800
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/62800
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1271510
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionA novel stationary phase prepared by the thermal immobilization of poly(dimethylsiloxane) onto the surface of silica (PDMS-SiO2) has been described, evaluated and compared with 229 commercially available RP-LC stationary phases using the Tanaka column classification protocol. The phase exhibited many unique chromatographic properties and, based on the phases in the database, was most similar to the fluoroalkylated phases (aside from the obvious lack of fluoro selectivity imposed by the C-F dipole). The phase exhibited classic reversed-phase behaviour in acid mobile phase conditions and mixed-mode reversed-phase/cation-exchange retention behaviour in neutral mobile phase conditions. The phase exhibited acceptable stability at both low and intermediate pH, conditions which should impart optimum chromatographic selectivity to the phase. Retention of basic analytes was shown to occur by a "three site model" as proposed by Neue. This new PDMS-SiO2 stationary phase is extremely interesting in that the dominancy of its hydrophobic and ion-exchange interactions can be controlled by the influence of mobile phase pH, buffer type and concentration. The PDMS-SiO2 stationary phase may provide a complementary tool to reversed-phase and HILIC stationary phases. The present results highlight the fact that the type of buffer, its concentration and pH can not only affect peak shape but also retention, selectivity and hence chromatographic resolution. Therefore, in method development and optimization strategies it is suggested that more emphasis should be given to the evaluation of these mobile phase operating parameters especially when basic solutes are involved.
dc.description402
dc.description6
dc.description2043
dc.description2055
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.languageen
dc.publisherSpringer Heidelberg
dc.publisherHeidelberg
dc.publisherAlemanha
dc.relationAnalytical And Bioanalytical Chemistry
dc.relationAnal. Bioanal. Chem.
dc.rightsfechado
dc.rightshttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.sourceWeb of Science
dc.subjectMixed-mode stationary phase
dc.subjectReversed-phase/cation-exchange stationary phase
dc.subjectImmobilized polysiloxane
dc.subjectChromatographic test
dc.subjectChemometrics
dc.subjectBasic solutes
dc.subjectPerformance Liquid-chromatography
dc.subjectPrincipal Component Analysis
dc.subjectIonizable Compounds
dc.subjectBasic Compounds
dc.subjectMobile-phase
dc.subjectSilanol Activity
dc.subjectPh Variation
dc.subjectRetention
dc.subjectColumns
dc.subjectSelectivity
dc.titleCharacterization of a mixed-mode reversed-phase/cation-exchange stationary phase prepared by thermal immobilization of poly(dimethylsiloxane) onto the surface of silica
dc.typeArtículos de revistas


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