Artículos de revistas
Virus inactivation by anilinonaphthalene sulfonate compounds and comparison with other ligands
Registro en:
Biochemical And Biophysical Research Communications. Academic Press Inc, v. 275, n. 3, n. 955, n. 961, 2000.
0006-291X
WOS:000089264900040
10.1006/bbrc.2000.3402
Autor
Bonafe, CFS
Glaser, M
Voss, EW
Weber, G
Silva, JL
Institución
Resumen
Bis-(8-anilinonaphthalene-1-sulfonate) (bis-ANS) causes inactivation of vesicular stomatitis virus (VSV) at micromolar concentrations while butyl-ANS and ANS are effective at concentrations one and two orders of magnitude higher, respectively. VSV fully inactivated by the combined effects of 10 mu M bis-ANS and 2.5 kbar hydrostatic pressure elicited a high titer of neutralizing antibodies. Incubation of VSV with greater than or equal to 2 M urea at atmospheric pressure caused very little virus inactivation, whereas at a pressure of 2.5 kbar, 1 M urea caused inactivation that exceeded by more than two orders of magnitude the sum of the inactivating effects produced by urea and pressure separately. Measurements of bis-ANS fluorescence showed that increasing the urea concentration reduces the pressure required to disrupt the structure. We conclude that anilinonaphthalene sulfonate compounds inactivate VSV by a mechanism similar to that produced by pressure. The most effective antiviral compound was bis-ANS which can be used for the preparation of safe viral vaccines or as an antiviral drug eventually. (C) 2000 Academic Press. 275 3 955 961