Virus inactivation by anilinonaphthalene sulfonate compounds and comparison with other ligands

dc.creatorBonafe, CFS
dc.creatorGlaser, M
dc.creatorVoss, EW
dc.creatorWeber, G
dc.creatorSilva, JL
dc.date2000
dc.dateSEP 7
dc.date2014-12-02T16:27:35Z
dc.date2015-11-26T16:34:32Z
dc.date2014-12-02T16:27:35Z
dc.date2015-11-26T16:34:32Z
dc.date.accessioned2018-03-28T23:16:46Z
dc.date.available2018-03-28T23:16:46Z
dc.identifierBiochemical And Biophysical Research Communications. Academic Press Inc, v. 275, n. 3, n. 955, n. 961, 2000.
dc.identifier0006-291X
dc.identifierWOS:000089264900040
dc.identifier10.1006/bbrc.2000.3402
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/76553
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/76553
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/76553
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1271224
dc.descriptionBis-(8-anilinonaphthalene-1-sulfonate) (bis-ANS) causes inactivation of vesicular stomatitis virus (VSV) at micromolar concentrations while butyl-ANS and ANS are effective at concentrations one and two orders of magnitude higher, respectively. VSV fully inactivated by the combined effects of 10 mu M bis-ANS and 2.5 kbar hydrostatic pressure elicited a high titer of neutralizing antibodies. Incubation of VSV with greater than or equal to 2 M urea at atmospheric pressure caused very little virus inactivation, whereas at a pressure of 2.5 kbar, 1 M urea caused inactivation that exceeded by more than two orders of magnitude the sum of the inactivating effects produced by urea and pressure separately. Measurements of bis-ANS fluorescence showed that increasing the urea concentration reduces the pressure required to disrupt the structure. We conclude that anilinonaphthalene sulfonate compounds inactivate VSV by a mechanism similar to that produced by pressure. The most effective antiviral compound was bis-ANS which can be used for the preparation of safe viral vaccines or as an antiviral drug eventually. (C) 2000 Academic Press.
dc.description275
dc.description3
dc.description955
dc.description961
dc.languageen
dc.publisherAcademic Press Inc
dc.publisherSan Diego
dc.publisherEUA
dc.relationBiochemical And Biophysical Research Communications
dc.relationBiochem. Biophys. Res. Commun.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectvesicular stomatitis virus
dc.subjectpressure inactivation
dc.subjectbis-ANS
dc.subjectHydrostatic-pressure
dc.subjectMosaic-virus
dc.subjectCoat Protein
dc.subjectDissociation
dc.subjectStability
dc.subjectBacteriophage
dc.subjectFluorescence
dc.subjectAssociation
dc.subjectDependence
dc.subjectComponents
dc.titleVirus inactivation by anilinonaphthalene sulfonate compounds and comparison with other ligands
dc.typeArtículos de revistas


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