Artículos de revistas
Amelogenin- and enamelysin (Mmp-20)-deficient mice display altered birefringence in the secretory-stage enamel organic extracellular matrix
Registro en:
Connective Tissue Research. Informa Healthcare, v. 48, n. 1, n. 39, n. 45, 2007.
0300-8207
1607-8438
WOS:000244529400006
10.1080/03008200601059175
Autor
Santo, ARE
Bartlett, JD
Gibson, CW
Li, Y
Kulkarni, AB
Line, SRP
Institución
Resumen
Dental enamel is the most mineralized tissue of vertebrate organisms. Enamel biosynthesis is initiated by the secretion, processing, and self-assembly of a complex mixture of proteins. The formation of an ordered enamel organic extracellular matrix (ECM) seems be a crucial step for the proper formation of mineral phase. Polarizing microscopy demonstrates that the ordered supramolecular structure of the secretory-stage enamel organic ECM is strongly birefringent. In the present work we analyzed the birefringence of secretory-stage enamel organic ECM in amelogenin (Amelx)- and enamelysin (Mmp20)-deficient mice. Female Amelx(+/-) animals showed significant reduction in optical retardation values when compared with the Amelx(+/+) subgroup (p = 0.0029). The secretory-stage enamel organic ECM of the Amelx(-/-) subgroup did not exhibit birefringence. The secretory-stage enamel organic ECM of Mmp20(-/-) mice showed a significant decrease in optical retardation as compared with Mmp20(+/+) and Mmp20(+/-) mice (p = 0.0000). Minp20(+/-) and Mmp20(+/+) mice exhibited similar birefringence (p = 1.0000). The results presented here support growing evidence for the idea that the birefringence of secretory-stage enamel organic ECM is influenced by the ordered supramolecular organization of its components. 48 1 39 45