Amelogenin- and enamelysin (Mmp-20)-deficient mice display altered birefringence in the secretory-stage enamel organic extracellular matrix

dc.creatorSanto, ARE
dc.creatorBartlett, JD
dc.creatorGibson, CW
dc.creatorLi, Y
dc.creatorKulkarni, AB
dc.creatorLine, SRP
dc.date2007
dc.date2014-11-13T19:35:00Z
dc.date2015-11-26T16:02:18Z
dc.date2014-11-13T19:35:00Z
dc.date2015-11-26T16:02:18Z
dc.date.accessioned2018-03-28T22:51:44Z
dc.date.available2018-03-28T22:51:44Z
dc.identifierConnective Tissue Research. Informa Healthcare, v. 48, n. 1, n. 39, n. 45, 2007.
dc.identifier0300-8207
dc.identifier1607-8438
dc.identifierWOS:000244529400006
dc.identifier10.1080/03008200601059175
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/77311
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/77311
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/77311
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1265032
dc.descriptionDental enamel is the most mineralized tissue of vertebrate organisms. Enamel biosynthesis is initiated by the secretion, processing, and self-assembly of a complex mixture of proteins. The formation of an ordered enamel organic extracellular matrix (ECM) seems be a crucial step for the proper formation of mineral phase. Polarizing microscopy demonstrates that the ordered supramolecular structure of the secretory-stage enamel organic ECM is strongly birefringent. In the present work we analyzed the birefringence of secretory-stage enamel organic ECM in amelogenin (Amelx)- and enamelysin (Mmp20)-deficient mice. Female Amelx(+/-) animals showed significant reduction in optical retardation values when compared with the Amelx(+/+) subgroup (p = 0.0029). The secretory-stage enamel organic ECM of the Amelx(-/-) subgroup did not exhibit birefringence. The secretory-stage enamel organic ECM of Mmp20(-/-) mice showed a significant decrease in optical retardation as compared with Mmp20(+/+) and Mmp20(+/-) mice (p = 0.0000). Minp20(+/-) and Mmp20(+/+) mice exhibited similar birefringence (p = 1.0000). The results presented here support growing evidence for the idea that the birefringence of secretory-stage enamel organic ECM is influenced by the ordered supramolecular organization of its components.
dc.description48
dc.description1
dc.description39
dc.description45
dc.languageen
dc.publisherInforma Healthcare
dc.publisherLondon
dc.publisherInglaterra
dc.relationConnective Tissue Research
dc.relationConnect. Tissue Res.
dc.rightsfechado
dc.rightshttp://informahealthcare.com/userimages/ContentEditor/1255620309227/Copyright_And_Permissions.pdf
dc.sourceWeb of Science
dc.subjectamelogenin
dc.subjectbirefringence
dc.subjectenamel organic extracellutar matrix
dc.subjectenamelysin
dc.subjectknockout
dc.subjectDeveloping Dental Enamel
dc.subjectHydroxyapatite Crystals
dc.subjectRecombinant Amelogenin
dc.subjectImperfecta Phenotype
dc.titleAmelogenin- and enamelysin (Mmp-20)-deficient mice display altered birefringence in the secretory-stage enamel organic extracellular matrix
dc.typeArtículos de revistas


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