The effects of cadmium chloride (CdCl2) on oxidative stress in the skeletal muscle cell line C2C12 were investigated. Myoblast cells that differentiated into myotubes were treated with CdCl 2 (1, 3, 5, 7.5, 10, and 12.5 μM) for 24, 48, and 72 h. Subsequent assay of cell homogenates for MTT (3-(4,5-dimethylthiozol-2-yl)-2,5- diphenyltetrazolium bromide) reduction, neutral red uptake and nucleic acid content showed that cadmium was toxic to C2C12 cells in a concentration-dependent manner. Glutathione-S-transferase activity (nmol μg of protein-1 min-1) was increased with 1 and 3 μM CdCl2 (36.9 ± 5.6 and 32.1 ± 6.0, respectively) compared to control cells (21.8 ± 1.5), but decreased at higher concentrations (7.5 μM = 15.9 ± 3.3, 10 μM = 15.9 ± 4.6, and 12.5 μM = 10.5 ± 2.8). 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