| dc.creator | Yano C.L. | |
| dc.creator | Marcondes M.C.C.G. | |
| dc.date | 2005 | |
| dc.date | 2015-06-26T14:07:48Z | |
| dc.date | 2015-11-26T15:42:08Z | |
| dc.date | 2015-06-26T14:07:48Z | |
| dc.date | 2015-11-26T15:42:08Z | |
| dc.date.accessioned | 2018-03-28T22:50:41Z | |
| dc.date.available | 2018-03-28T22:50:41Z | |
| dc.identifier | | |
| dc.identifier | Free Radical Biology And Medicine. , v. 39, n. 10, p. 1378 - 1384, 2005. | |
| dc.identifier | 8915849 | |
| dc.identifier | 10.1016/j.freeradbiomed.2005.07.001 | |
| dc.identifier | http://www.scopus.com/inward/record.url?eid=2-s2.0-27544490827&partnerID=40&md5=7a2a2c977cb7f15f38107664692e8023 | |
| dc.identifier | http://www.repositorio.unicamp.br/handle/REPOSIP/93435 | |
| dc.identifier | http://repositorio.unicamp.br/jspui/handle/REPOSIP/93435 | |
| dc.identifier | 2-s2.0-27544490827 | |
| dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1264782 | |
| dc.description | The effects of cadmium chloride (CdCl2) on oxidative stress in the skeletal muscle cell line C2C12 were investigated. Myoblast cells that differentiated into myotubes were treated with CdCl 2 (1, 3, 5, 7.5, 10, and 12.5 μM) for 24, 48, and 72 h. Subsequent assay of cell homogenates for MTT (3-(4,5-dimethylthiozol-2-yl)-2,5- diphenyltetrazolium bromide) reduction, neutral red uptake and nucleic acid content showed that cadmium was toxic to C2C12 cells in a concentration-dependent manner. Glutathione-S-transferase activity (nmol μg of protein-1 min-1) was increased with 1 and 3 μM CdCl2 (36.9 ± 5.6 and 32.1 ± 6.0, respectively) compared to control cells (21.8 ± 1.5), but decreased at higher concentrations (7.5 μM = 15.9 ± 3.3, 10 μM = 15.9 ± 4.6, and 12.5 μM = 10.5 ± 2.8). An increase in malondialdehyde content (nmol μg of protein-1), especially at high CdCl2 concentrations (control = 7.3 ± 0.5; CdCl2: 7.5 μM = 11.2 ± 3.1, 10 μM = 14.6 ± 3.8, and 12.5 μM = 20.5 ± 6.5) indicated that there was enhanced lipid peroxidation. Light and scanning electron microscopy showed that there was a concentration-dependent loss of adherent cells and the formation of vesicles indicative of cell death. These results indicated that CdCl2 increased oxidative stress in C 2C12 cells, and this stress probably compromised cell adhesion and the cellular antioxidant defense mechanisms. | |
| dc.description | 39 | |
| dc.description | 10 | |
| dc.description | 1378 | |
| dc.description | 1384 | |
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| dc.language | en | |
| dc.publisher | | |
| dc.relation | Free Radical Biology and Medicine | |
| dc.rights | fechado | |
| dc.source | Scopus | |
| dc.title | Cadmium Chloride-induced Oxidative Stress In Skeletal Muscle Cells In Vitro | |
| dc.type | Artículos de revistas | |
