Artículos de revistas
A Trypsin Inhibitor From Peltophorum Dubium Seeds Active Against Pest Proteases And Its Effect On The Survival Of Anagasta Kuehniella (lepidoptera: Pyralidae)
Registro en:
Biochimica Et Biophysica Acta - General Subjects. , v. 1621, n. 2, p. 170 - 182, 2003.
3044165
10.1016/S0304-4165(03)00055-2
2-s2.0-0242500472
Autor
Rodrigues Macedo M.L.
Freire M.D.G.M.
Cabrini E.C.
Toyama M.H.
Novello J.C.
Marangoni S.
Institución
Resumen
A novel trypsin inhibitor was purified from the seeds of Peltophorum dubium (Spreng.). SDS-PAGE under reducing conditions showed that the inhibitor consisted of a single polypeptide chain (ca. 20 kDa). The dissociation constants of 4×10-10 and 1.6×10-10 M were obtained with bovine and porcine trypsin, respectively. This constant was lower (2.6×10-7 M) for chymotrypsin. The inhibitory activity was stable over a wide range of temperature and pH and in the presence of DTT. The N-terminal sequence of the P. dubium inhibitor showed a high degree of homology with other Kunitz-type inhibitors. When fed to the insect Anagasta kuehniella, in an artificial diet (inhibitor concentration 1.6%), the inhibitor produced ∼56% and delayed the development of this lepidopteran. The concentration of inhibitor in the diet necessary to cause a 50% reduction in the weight (ED50) of fourth instar larvae was ∼1%. The action of the P. dubium trypsin inhibitor (PDTI) on A. kuehniella may involve inhibition of the trypsin-like activity present in the larval midgut, resistance of the inhibitor to digestion by midgut enzymes and bovine trypsin, and association of the inhibitor with a chitin column and chitinous structures in the peritrophic membrane and/or midgut of the insect. © 2003 Elsevier Science B.V. All rights reserved. 1621 2 170 182 Richardson, M., Seed storage proteins: The enzyme inhibitors (1991) Methods in Plant Biochemistry, Amino Acids, Proteins and Nucleic Acids, 5, pp. 259-305. , Rogers J.L. New York: Academic Press Ryan, C.A., Protease inhibitors in plants: Genes for improving defenses against insects and pathogens (1990) Annu. Ver. Phytopathol., 28, pp. 425-449 Shewry, P.R., Lucas, J.A., Plant proteins that confer resistance to pests and pathogens (1997) Advances in Botanical Research, 26, pp. 136-192. , Callow J. 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