A Trypsin Inhibitor From Peltophorum Dubium Seeds Active Against Pest Proteases And Its Effect On The Survival Of Anagasta Kuehniella (lepidoptera: Pyralidae)

dc.creatorRodrigues Macedo M.L.
dc.creatorFreire M.D.G.M.
dc.creatorCabrini E.C.
dc.creatorToyama M.H.
dc.creatorNovello J.C.
dc.creatorMarangoni S.
dc.date2003
dc.date2015-06-30T17:29:04Z
dc.date2015-11-26T15:41:02Z
dc.date2015-06-30T17:29:04Z
dc.date2015-11-26T15:41:02Z
dc.date.accessioned2018-03-28T22:49:31Z
dc.date.available2018-03-28T22:49:31Z
dc.identifier
dc.identifierBiochimica Et Biophysica Acta - General Subjects. , v. 1621, n. 2, p. 170 - 182, 2003.
dc.identifier3044165
dc.identifier10.1016/S0304-4165(03)00055-2
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-0242500472&partnerID=40&md5=f58df18a6f04c217781b0c21315578ff
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/102226
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/102226
dc.identifier2-s2.0-0242500472
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1264519
dc.descriptionA novel trypsin inhibitor was purified from the seeds of Peltophorum dubium (Spreng.). SDS-PAGE under reducing conditions showed that the inhibitor consisted of a single polypeptide chain (ca. 20 kDa). The dissociation constants of 4×10-10 and 1.6×10-10 M were obtained with bovine and porcine trypsin, respectively. This constant was lower (2.6×10-7 M) for chymotrypsin. The inhibitory activity was stable over a wide range of temperature and pH and in the presence of DTT. The N-terminal sequence of the P. dubium inhibitor showed a high degree of homology with other Kunitz-type inhibitors. When fed to the insect Anagasta kuehniella, in an artificial diet (inhibitor concentration 1.6%), the inhibitor produced ∼56% and delayed the development of this lepidopteran. The concentration of inhibitor in the diet necessary to cause a 50% reduction in the weight (ED50) of fourth instar larvae was ∼1%. The action of the P. dubium trypsin inhibitor (PDTI) on A. kuehniella may involve inhibition of the trypsin-like activity present in the larval midgut, resistance of the inhibitor to digestion by midgut enzymes and bovine trypsin, and association of the inhibitor with a chitin column and chitinous structures in the peritrophic membrane and/or midgut of the insect. © 2003 Elsevier Science B.V. All rights reserved.
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dc.languageen
dc.publisher
dc.relationBiochimica et Biophysica Acta - General Subjects
dc.rightsfechado
dc.sourceScopus
dc.titleA Trypsin Inhibitor From Peltophorum Dubium Seeds Active Against Pest Proteases And Its Effect On The Survival Of Anagasta Kuehniella (lepidoptera: Pyralidae)
dc.typeArtículos de revistas


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