Artículos de revistas
The Thiol-specific Antioxidant Enzyme Prevents Mitochondrial Permeability Transition: Evidence For The Participation Of Reactive Oxygen Species In This Mechanism
Registro en:
Journal Of Biological Chemistry. , v. 273, n. 21, p. 12766 - 12769, 1998.
219258
10.1074/jbc.273.21.12766
2-s2.0-0032557424
Autor
Kowaltowski A.J.
Netto L.E.S.
Vercesi A.E.
Institución
Resumen
Mitochondrial swelling and membrane protein thiol oxidation associated with mitochondrial permeability transition induced by Ca2+ and inorganic phosphate are inhibited in a dose-dependent manner either by catalase, the thiol-specific antioxidant enzyme (TSA), a protein recently demonstrated to present thiol peroxidase activity, or ebselen, a selenium-containing heterocycle which also possesses thiol peroxidase activity. This inhibition of mitochondrial permeability transition is due to the removal of mitochondrial-generated H2O2 which can easily diffuse to the extramitochondrial space. Whereas ebselen required the presence of reduced glutathione as a reductant to grant its protective effect, TSA was fully reduced by mitochondrial components. Decrease in the oxygen concentration of the reaction medium also inhibits mitochondrial permeabilization and membrane protein thiol oxidation, in a concentration-dependent manner. The results presented in this report confirm that mitochondrial permeability transition induced by Ca2+ and inorganic phosphate is reactive oxygen species- dependent. The possible importance of TSA as an intracellular antioxidant, avoiding the onset of mitochondrial permeability transition, is discussed in the text. 273 21 12766 12769 Gunter, T.E., Gunter, K.K., Sheu, S.-S., Gavin, C.E., (1994) Am. J. Physiol., 267, pp. C313-C339 Zoratti, M., Szabó, I., (1995) Biochim. Blophys. Acta, 1241, pp. 139-176 Lehninger, A.L., Vercesi, A.E., Bababunmi, E.A., (1978) Proc. Natl. Acad. Sci. U. S. A., 79, pp. 6842-6846 Vercesi, A.E., Kowaltowski, A.J., Grijalba, M.T., Meinicke, A.R., Castilho, R.F., (1997) Biosci. Rep., 17, pp. 43-52 Castilho, R.F., Kowaltowski, A.J., Meinicke, A.R., Vercesi, A.E., (1995) Free Radical Biol. & Med., 18, pp. 479-486 Kowaltowski, A.J., Castilho, R.F., Vercesi, A.E., (1995) Am. J. Physiol., 269, pp. C141-C147 Valle, V.G.R., Fagian, M.M., Parentoni, L.S., Meinicke, A.R., Vercesi, A.E., (1993) Arch. Biockem. Biophys., 307, pp. 1-7 Kowaltowski, A.J., Castilho, R.F., Vercesi, A.E., (1996) FEBS Lett., 378, pp. 150-152 Kowaltowski, A.J., Castilho, R.F., Grijalba, M.T., Bechara, E.J.H., Vercesi, A.E., (1996) J. Biol. Chem., 271, pp. 2929-2934 Fagian, M.M., Pereira-da-Silva, L., Martins, I.S., Vercesi, A.E., (1990) J. Biol. Chem., 265, pp. 19955-19960 Castilho, R.F., Kowaltowski, A.J., Vercesi, A.E., (1996) J. Bioenerg. Biomembr., 28, pp. 523-529 Scorrano, L., Petronilli, V., Bernardi, P., (1997) J. Biol. Chem., 272, pp. 12295-12299 Pastorino, J.G., Snyder, J.W., Serroni, A., Hoek, J.B., Farber, J.L., (1993) J. Biol. Chem., 268, pp. 13791-13798 Griffiths, E., Halestrap, A.P., (1995) Biochem. J., 307, pp. 93-98 Zamzami, N., Hirsch, T., Dallaporta, B., Petit, P.X., Kroemer, G., (1997) J. Bioenerg. Biomembr., 29, pp. 185-193 Skulachev, V.P., (1996) FEBS Lett., 397, pp. 7-10 Kim, K., Kim, I.H., Lee, K.-Y., Rhee, S.G., Stadtman, E.R., (1988) J. Biol. Chem., 263, pp. 4704-4711 Chae, H.Z., Robison, K., Poole, L.B., Church, G., Storz, G., Rhee, S.G., (1994) Proc. Natl. Acad. Sci. U. S. A., 91, pp. 7017-7021 Chae, H.Z., Chung, S.J., Rhee, S.G., (1994) J. Biol. Chem., 269, pp. 27670-27678 Netto, L.E.S., Chae, H.Z., Kang, S.-W., Rhee, S.G., Stadtman, E.R., (1996) J. Biol. Chem., 271, pp. 15315-15321 Nogoceke, E., Gommel, D.U., Kieb, M., Kalisz, H.M., Flohé, L., (1997) Biol. Chem., 378, pp. 827-836 Kim, I.H., Kim, K., Rhee, S.G., (1989) Proc. Natl. Acad. Sci. U. S. A., 86, pp. 6018-6022 Watabe, S., Kohno, H., Kouyama, H., Hiroi, T., Yago, N., Nakazawa, T., (1994) J. Biochem. (Tokyo), 115, pp. 648-654 Ishii, T., Kawane, T., Taketani, S., Bannai, S., (1995) Biochem. Biophys. Res. Commun., 216, pp. 970-975 Sies, H., (1993) Free Radical Biol. & Med., 14, pp. 313-323 Kowaltowski, A.J., Vercesi, A.E., Castilho, R.F., (1997) Biochim. Biophys. Acta, 1318, pp. 385-402 Boveris, A., Martino, E., Stoppani, A.O.M., (1977) Anal. Biochem., 80, pp. 145-158 Chae, H.Z., Uhm, T.B., Rhee, S.G., (1994) Proc. Natl. Acad. Sci. U. S. A., 91, pp. 7022-7026 Tsuji, K., Copeland, N.G., Jenkins, N.A., Obinata, M., (1995) Biochem. J., 307, pp. 377-381 Zhang, P., Liu, B., Kang, S.W., Seo, M.S., Rhee, S.G., Obeid, L.M., (1997) J. Biol. Chem., 272, pp. 30615-30618