Artículos de revistas
Gene Expression Profiling In Genetic Animal Models Of Provide Elements To Unveil The Molecular Mechanisms Underlying Epileptogenesis In Rodents [a Caracterização Do Perfil De Expressão Gênica Em Larga Escala Em Modelos Genéticos De Epilepsia Fornece Elementos Para Entender Os Mecanismos Envolvidos Na Epileptogênese Em Roedores]
Registro en:
Journal Of Epilepsy And Clinical Neurophysiology. , v. 18, n. 2, p. 50 - 52, 2012.
16762649
10.1590/S1676-26492012000200006
2-s2.0-84872563714
Autor
Matos A.H.B.
Pascoal V.D.B.
Nascimento D.R.
Martins S.
Rocha C.S.
Vasconcellos J.F.
Chamma M.T.
Maurer-Morelli C.V.
Martins A.S.
Valle A.C.
Godard A.L.B.
Lopes Cendes I.
Institución
Resumen
Objective: The objective of this study was to characterize and compare the genetic profile of two rodent models of epilepsy (Wistar Audiogenic Rat - WAR and rats with generalized epilepsy with absence seizures-GEAS) using gene expression analysis Methods: We used microarray technology for gene expression analysis. Results: The analysis of gene expression profiles in WAR showed among genes up-regulated Neurod1, involved in the development of the cochlear duct. In addition, we found significant differences in gene expression of Apbb1, Foxg1 and Scn1A. GEAS rats had differentially expressed genes related to the development of central nervous system, as well as genes involved in the MAPK pathway, transcription factors, neuronal migration and apoptosis. Conclusion: This study may help to clarify the underlying molecular mechanism that leads to the predisposition to seizures in these animals. Our results indicate the activation of distinct molecular pathways in both models. 18 2 50 52 Aicard, J., Course and prognosis of certain chidhood epilepsies with predominantly myoclonic seizures (1980) Advances in Epileptology. The Xth Epilepsy International Symposim, pp. 159-163. , Wada JA, Penry JK e cols. New York: Ravem André, E.S., Electrophysiological characterization of a new form of spontaneous epilepsy in Wistar rats (1999) Epilepsia, 40 (SUPPL. 2) Bruno-Neto, R., Caracterização de uma nova forma de epilepsia espontânea em ratos Wistar (1999) XIV FESBE, Anais Bruno-Neto, R., André, E.S., Pellarin, L., Hilário, F.K., Valle, A.C., Timo-Iaria, C., Electrophysiological characterization of a new form of spontaneous epilepsy in Wistar rats (1999) 23rd International Epilepsy Congress, Prague, Czech Republic Doretto, M.C., Fonseca, C.G., Lobo, R.B., Terra, V.C., Oliveira, J.A., Garcia-Cairasco, N., Quantitative study of the response to genetic selection of the WistarAudiogenic Rat strain (WAR) (2003) Behav Genet, 33 (1), pp. 33-42 Garcia-Cairasco, N., Sabbatini, R., Role of the substantianigra in audiogenic seizures: A neuroethological analysis in the rat (1983) Braz J Med Biol Res, 16 (2), pp. 171-183 Gitaí, D.L., Martinelli, H.N., Valente, V., Pereira, M.G., Oliveira, J.A., Elias, C.F., Bittencourt, J.C., Paçó-Larson, M.L., Increased expression of GluR2-flip in the hippocampus of the Wistaraudiogenic rat strain after acute and kindled seizures (2010) Hippocampus, 20, pp. 125-133 Jobe, P.C., Brown, R.D., Dailey, J.W., Effect of Ro 4-1284 on audiogenic seizure susceptibility and intensity in epilepsy-prone rats (1981) Life Sci, 28 (18), pp. 2031-2038 Kovács, Z., Suppression of spike-wave discharge activity and c-fos expression by2-methyl-4-oxo-3H-quinazoline-3-acetyl piperidine (Q5) in vivo (2007) Neuroscience Letters, 423, pp. 73-77 Mesquita, F., Aguiar, J.F., Oliveira, J.A., Garcia-Cairasco, N., Varanda, W.A., Electrophysiological properties of cultured hippocampal neurons from WistarAudiogenic rats (2005) Brain Res Bul, 65, pp. 177-183 Moraes, M.F., Galvis-Alonso, O.Y., Garcia-Cairasco, N., Audiogenic kindling in the Wistar rat: A potential model for recruitment of limbic structures (2000) Epilepsy Research, 39, pp. 251-259 Nunes, P.V., Valle, A.C., Timo-Iaria, C., Epileptogenic potentials recorded from the cerebellar cortex in rats (1999) Epilepsia, 40, p. 132. , 23th International Epilepsy Congress, Prague, Czech Republic