Recently, self-assemblies of peptide nanotubes (PNTs) have appeared as one of the most interesting nanostructures to be explored in the field of nanotechnology. These smart assemblies can have diverse applications, such as in the design of nanoreactors, sensors, electronics, and stimulus-responsive materials. Recent publications indicate that PNT synthesis and production are under extensive study. However, a more detailed safety and nanotoxicology evaluation of these materials is still necessary. This is of paramount importance since interesting and novel biomedical applications based on the use of PNTs, including the development of smart nanodevices and drug delivery systems, are under way. To this end, the aim of this mini-review is to discuss the recent biomedical applications of PNTs and, it hopes, to be a source of inspiration for researchers in different areas of expertise related to nanotechnology. © 2012 Elsevier Inc.3914754Adler-Abramovich, L., Reches, M., Sedman, V.L., Allen, S., Tendler, S.J.B., Gazit, E., Thermal and chemical stability of diphenylalanine peptide nanotubes: Implications for nanotechnological applications (2006) Langmuir, 22, pp. 1313-1320Aida, T., Meijer, E.W., Stupp, S.I., Functional supramolecular polymers (2012) Science, 335, pp. 813-817Andersen, K.B., Castillo-León, J., Bakmand, T., Svendsen, W.E., Alignment and use of self-assembled peptide nanotubes as dry-etching mask (2012) Japan J Appl Phys, 51, pp. 06FF13Andersen, K.B., Castillo-Leon, J., Hedstromb, M., Svendsen, W.E., Stability of diphenylalanine peptide nanotubes in solution (2011) Nanoscale, 3, pp. 994-998Banerjee, I.A., Yu, L., Matsui, H., Location-specific biological functionalization on nanotubes: Attachment of proteins at the ends of nanotubes using Au nanocrystal masks (2003) Nano Lett, 3, pp. 283-287Bong, D.T., Clark, T.D., Granja, J.R., Ghadiri, M.R., Self-assembling organic nanotubes (2001) Angew Chem Int Ed, 40, pp. 988-1011Brea, R.J., Herranz, M.A., Sanchez, L., Castedo, L., Seitz, W., Guldi, D.M., Electron transfer in Me-blocked heterodimeric alpha, gamma-peptide nanotubular donor-acceptor hybrids (2007) Proc Natl Acad Sci USA, 104, pp. 5291-5294Brea, R.J., Vazquez, M.E., Mosquera, M., Castedo, L., Granja, J.R., Controlling multiple fluorescent signal output in cyclic peptide-based supramolecular (2007) J Am Chem Soc, 129, pp. 1653-1657Castillo, J., Tanzi, S., Dimaki, M., Svendsen, W., Manipulation of self-assembly amyloid peptide nanotubes by dielectrophoresis (2008) Electrophoresis, 29, pp. 5026-5032Castillo-León, J., Rodriguez-Trujillo, R., Gauthier, S., Jensen, A.C.O., Svendsen, W.E., Micro-"factory" for self-assembled peptide nanostructures (2011) Microelectronic Eng, 88, pp. 1685-1688Clausen, C.H., Dimaki, M., Panagos, S.P., Kasotakis, E., Mitraki, A., Svendsen, W.E., Electrostatic force microscopy of self-assembled peptide structures (2011) Scanning, 33, pp. 201-207Clausen, C.H., Jensen, J., Castillo, J., Dimaki, M., Svendsen, W.E., Qualitative mapping of structurally different dipeptide nanotubes (2008) Nano Lett, 8, pp. 4066-4069Cipriano, T.C., Takahashi, P.M., De Lima, D., Oliveira, Jr.V.X., Souza, J.A., Martinho, H., Spatial organization of peptide nanotubes for electrochemical devices (2010) J Mater Sci, 45, pp. 5101-5108Chandra, S., De, K., Ganguly, S., Sarkar, B., Misra, M., Synthesis, radiolabeling and biological evaluation of a neutral tripeptide and its derivatives for potential nuclear medicine applications (2009) Peptides, 30, pp. 2399-2408Chen, C.X., Pan, F., Zhang, S.Z., Hu, J., Cao, M.W., Wang, J., Antibacterial activities of short designer peptides: A link between propensity for nanostructuring and capacity for membrane destabilization (2010) Biomacromolecules, 11, pp. 402-411Chow, L.W., Bitton, R., Webber, M.J., Carvajal, D.I., Shull, K.R., Sharma, A.K., A bioactive self-assembled membrane to promote angiogenesis (2011) Biomaterials, 32, pp. 1574-1582Couet, J., Samuel, J.D.J.S., Kopyshev, A., Santer, S., Biesalski, M., Peptide-polymer hybrid nanotubes (2005) Angew Chem Int Ed, 44, pp. 3297-3301Cui, H., Webber, M.J., Stupp, S.I., Self-assembly of peptide amphiphiles: From molecules to nanostructures to biomaterials (2010) Pept Sci, 94, pp. 1-18Dartois, V., Sanchez-Quesada, J., Cabezas, E., Chi, E., Dubbelde, C., Dunn, C., Systemic antibacterial activity of novel synthetic cyclic peptides (2005) Antimicrob Agents Chemother, 49, pp. 3302-3310De La Rica, R., Matsui, H., Applications of peptide and protein-based materials in bionanotechnology (2010) Chem Soc Rev, 39, pp. 3499-3509De La Rica, R., Mendoza, E., Lechuga, L.M., Matsui, H., Label-free pathogen detection with sensor chips assembled from peptide nanotubes (2008) Angew Chem Int Ed, 47, pp. 9752-9755Ellis-Behnke, R.G., Liang, Y.X., You, S.W., Tay, D.K., Zhang, S., So, K.F., Nano neuro knitting: Peptide nanofiber scaffold for brain repair and axon regeneration with functional return of vision (2006) Proc Natl Acad Sci USA, 103, pp. 5054-5059Fenniri, H., Deng, B.L., Ribbe, A.E., Hallenga, K., Jacob, J., Thiyagarajan, P., Entropically driven self-assembly of multichannel rosette nanotubes (2002) Proc Natl Acad Sci USA, 99, pp. 6487-6492Fenniri, H., Mathivanan, P., Vidale, K.L., Sherman, D.M., Hallenga, K., Wood, K.V., Helical rosette nanotubes: Design, self-assembly, and characterization (2001) J Am Chem Soc, 123, pp. 3854-3855Fernandez-Lopez, S., Kim, H.-S., Choi, E.C., Delgado, M., Granja, J.R., Khasanov, A., Antibacterial agents based on the cyclic D,L-alpha-peptide architecture (2001) Nature, 412, pp. 452-455Fletcher, J.T., Finlay, J.A., Callow, M.E., Callow, J.A., Ghadiri, M.R., A combinatorial approach to the discovery of biocidal six-residue cyclic D,L-alpha-peptides against the bacteria methicillin-resistant Staphylococcus aureus (MRSA) and E. coli and the biofouling algae Ulva linza and Navicula perminuta (2007) Chem Eur J, 13, pp. 4008-4013Ghadiri, M.R., Cyclic Peptide Tube, , US Patent 200320036613875Ghadiri, M.R., Granja, J.R., Buehler, L.K., Artificial transmembrane ion channels from self-assembling peptide nanotubes (1994) Nature, 369, pp. 301-304Ghadiri, M.R., Granja, J.R., Milligan, R.A., McRee, D.E., Kazanovich, N., Self-assembling organic nanotubes based on a cyclic peptide architecture (1993) Nature, 366, pp. 324-327Granja, J.R., Ghadiri, M.R., Channel-mediated transporto f glucose across lipid bilayers (1994) J Am Chem Soc, 116, pp. 10785-10786Gomes, S., Leonor, I.B., Mano, J.F., Reis, R.L., Kaplan, D.L., Natural and genetically engineered proteins for tissue engineering (2012) Prog Polym Sci, 37, pp. 1-17Hartgerink, J.D., Clark, T.D., Ghadiri, M.R., Peptide nanotubes and beyond (1998) Chem Eur J, 4, pp. 1367-1372Hartgerink, J.D., Granja, J.R., Milligan, R.A., Ghadiri, M.R., Self-assembling peptide nanotubes (1996) J Am Chem Soc, 118, pp. 43-50Horne, W.S., Wiethoff, C.M., Cui, C., Wilcoxen, K.M., Amorin, M., Ghadiri, M.R., Antiviral cyclic D,L-alpha-peptides: Targeting a general biochemical pathway in virus infections (2005) Bioorg Med Chem, 13, pp. 5145-5153Hsieh, W.H., Chang, S.F., Chen, H.M., Chen, J.H., Liaw, J., Oral gene delivery with cyclo-(D-Trp-Tyr) peptide nanotubes (2012) Mol Pharm, 9, pp. 1231-1249Johnson, K.T., Fath, K.R., Henricus, M.M., Banerjee, I.A., Self-assembly and growth of smart cell-adhesive mucin bound microtubes (2009) Soft Matter, 7, pp. 21-36Kim, J.H., Ryu, J., Park, C.B., Selective detection of neurotoxin by photoluminescent peptide nanotubes (2011) Small, 7, pp. 718-722Kanlayavattanakul, M., Lourith, N., Lipopeptides in cosmetics (2010) Int J Cosmet Sci, 32, pp. 1-8Komatsu, T., Terada, H., Kobayashi, N., Protein nanotubes with an enzyme interior surface (2011) Chem Eur J, 17, pp. 1849-1854Lakshmanan, A., Zhang, S., Hauser, C.A.E., Short self-assembling peptides as building blocks for modern nanodevices (2012) Trends Biotechnol, 30, pp. 155-165Laromaine, A., Koh, L.L., Murugesan, M., Ulijn, R.V., Stevens, M.M., Protease-triggered dispersion of nanoparticle assemblies (2007) J Am Chem Soc, 129, p. 4156Loo, Y., Zhang, S., Hauser, C.A.E., From short peptides to nanofibers to macromolecular assemblies in biomedicine (2012) Biotechnol Adv, 30, pp. 593-603Mata, A., Hsu, L., Capito, R., Aparicio, C., Henriksonc, K., Stupp, S.I., Micropatterning of bioactive self-assembling gels (2009) Soft Matter, 5, pp. 1228-1236Matsui, H., MacCuspie, R., Metalloporphyrin nanotube fabrication using peptide nanotubes as templates (2001) Nano Lett, 1, pp. 671-675McGimpsey, W.G., Cyclic Peptide Structures for Molecular Scale Electronic and Photonic Devices, , US Patent 200320030144185A1McGimpsey, W.G., Cyclic Peptide Nanotube Structures for Molecular Scale Electronic and Photonic Devices, , US Patent 200520050124535A1McGimpsey, W.G., Grant, W., Cyclic Peptide Structures for Molecular Scale Electronic and Photonic Devices, , US Patent 200520056902720Mitraki, A., Papanikolopoulou, K., Van Raaij, M.J., Natural triple beta-stranded fibrous folds (2006) Adv Protein Chem, 73, pp. 97-124Motesharei, K., Ghadiri, M.R., Diffusion-limited size-selective ion sensing based on SAM-supported peptide nanotubes (1997) J Am Chem Soc, 119, pp. 11306-11312Nuraje, N., Banerjee, I.A., MacCuspie, R.I., Yu, L., Matsui, H., Biological bottom-up assembly of antibody nanotubes on patterned antigen arrays (2004) J Am Chem Soc, 126, pp. 8088-8089Park, B.W., Ko, K.A., Yoon, D.Y., Kim, D.S., Enzyme activity assay for horseradish peroxidase encapsulated in peptide nanotubes (2012) Enzyme Microb Technol, 51, pp. 81-85Park, J.H., Kwon, S., Nam, J.O., Park, R.W., Chung, H., Seo, S.B., Self-assembled nanoparticles based on glycol chitosan bearing 5h-cholanic acid for RGD peptide delivery (2004) J Control Release, 95, pp. 579-588Petrov, A., Audette, G.F., Peptide and protein-based nanotubes for nanobiotechnology (2012) WIREs Nanomed Nanobiotechnol, 4, pp. 575-585. , 10.1002/wnan.1180Pouget, R., Fay, N., Dujardin, E., Jamin, N., Berthault, P., Perrin, L., Elucidation of the self-assembly pathway of lanreotide octapeptide into β-Sheet (2010) J Am Chem Soc, 132, pp. 4230-4241Prodan, M., Ionita, D., Ungureanu, C., Bojin, D., Demetrescu, I., Enhancing antibacterial effect of multiwalled carbon nanotubes using silver nanoparticles (2011) Dig J Nanomat Biostruct, 6, pp. 549-556Qu, X., Kobayashi, N., Komatsu, T., Solid nanotubes comprising α-Fe2O3 nanoparticles prepared from ferritin protein (2010) ACS Nano, 4, pp. 1732-1738Qu, X., Komatsu, T., Molecular capture in protein nanotubes (2010) ACS Nano, 4, pp. 563-573Rahmat, F., Thamwattana, N., Cox, B.J., Modelling peptide nanotubes for artificial ion channels (2011) Nanotechnology, 22, p. 445707. , 10.1088/0957-4484/22/44/445707Rajangam, K., Bechanna, H.A., Hui, M.J., Han, X.Q., Hulvat, J.F., Lomasney, J.W., Heparin binding nanostructures to promote growth of blood vessels (2006) Nano Lett, 6, pp. 2086-2090Reches, M., Gazit, E., Casting metal nanowires within discrete self-assembled peptide nanotubes (2003) Science, 300, pp. 625-627Reches, M., Gazit, E., Formation of closed-cage nanostructures by self-assembly of aromatic dipeptides (2004) Nano Lett, 4, pp. 581-585Reches, M., Gazit, E., Peptide Nanostructures Containing End-capping Modified Peptides and Methods of Generating and Using the Same, , US Patent 201020100291828Reches, M., Gazit, E., Peptide Nanostructures and Methods of Generating and Using the Same. US Patent 2011, , 20090123553, AT 509886Rosenman, G., Beker, P., Koren, I., Yevnin, M., Bank-Srour, B., Mishina, E., Bioinspired peptide nanotubes: Deposition technology, basic physics and nanotechnology applications (2011) J Pept Sci, 17, pp. 75-87Ryu, J., Park, C.H., High stability of self-assembled peptide nanowires against thermal, chemical, and proteolytic attacks (2010) Biotechnol Bioeng, 105, pp. 221-230Sanchez-Quesada, J., Bayley, H., Ghadiri, M.R., Braha, O., Cyclic peptides as molecular adapters for a pore-forming protein (2000) J Am Chem Soc, 122, pp. 11757-11766Sanchez-Quesada, J., Kim, H.S., Ghadiri, M.R., A synthetic pore-mediated transmembrane transport of glutamic acid (2001) Angew Chem Int Ed, 40, p. 2503Scanlon, S., Aggeli, A., Self-assembling peptide nanotubes (2008) Nanotoday, 3, pp. 22-30Scheibel, T., Parthasarathy, R., Sawicki, G., Lin, X., Jaeger, H., Lindquist, S.L., Conducting nanowires built by controlled self-assembly of amyloid fibers and selective metal deposition (2003) Proc Natl Acad Sci USA, 100, pp. 4527-4532Sedman, V.L., Adler-Abramovich, L., Allen, S., Gazit, E., Tendler, S.J.B., Direct observation of the release of phenylalanine from diphenylalanine nanotubes (2006) J Am Chem Soc, 128, pp. 6903-6908Shekhar, S., Anjia, L., Matsui, H., Khondaker, S.I., Electrical transport properties of peptide nanotubes coated with gold nanoparticles via peptide-induced biomineralization (2011) Nanotechnology, 22, p. 095202Shimizu, T., Masuda, M., Minamikawa, H., Supramolecular nanotube architectures based on amphiphilic molecules (2005) Chem Rev, 105, pp. 1401-1403Song, Y., Challa, S.R., Medforth, C.J., Qiu, Y., Watt, R.K., Pena, D., Synthesis of peptide-nanotube platinum-nanoparticle composites (2004) Chem Commun, pp. 1044-1045Suri, S.S., Fenniri, H., Singh, B., Nanotechnology-based drug delivery systems (2007) J Occup Med Toxicol, 2, p. 16. , 10.1186/1745-6673-2-16Tarabout, C., Roux, S., Gobeaux, F., Fay, N., Pouget, E., Meriadec, C., Control of peptide nanotube diameter by chemical modifications of an aromatic residue involved in a single close contact (2011) Proc Natl Acad Sci USA, 108, pp. 7679-7684. , 10.1073/pnas.1017343108Ulijn, R.V., Smith, A.M., Designing peptide based nanomaterials (2008) Chem Soc Rev, 37, pp. 664-675Valery, C., Artzner, F., Paternostre, M., Peptide nanotubes: Molecular organizations, self-assembly mechanisms and applications (2011) Soft Matter, 7, pp. 9583-9594. , 10.1039/c1sm05698kWebber, M.J., Tongers, J., Newcomb, C.J., Marquardt, K.T., Bauersachs, J., Losordo, D.W., Supramolecular nanostructures that mimic VEGF as a strategy for ischemic tissue repair (2011) Proc Natl Acad Sci USA, 108, pp. 13438-13443. , 10.1073/pnas.1016546108Yan, X., He, Q., Wang, K., Duan, L., Cui, Y., Li, J., Transition of cationic dipeptide nanotubes into vesicles and oligonucleotide delivery (2007) Angew Chem Int Ed, 46, pp. 2431-2434Yan, X., Zhu, P., Li, J., Self-assembly and application of diphenylalanine-based nanostructures (2010) Chem Soc Rev, 39, pp. 1877-1890Yemini, M., Reches, M., Rishpon, J., Gazit, E., Novel electrochemical biosensing platform using self-assembled peptide nanotubes (2005) Nano Lett, 5, pp. 183-186Yemini, M., Reches, M., Rishpon, J., Gazit, E., YPeptide Nanostructure-coated Electrodes, , US Patent 200720070138007Yu, L., Banerjee, A.I.A., Gao, X., Nuraje, N., Matsui, H., Fabrication and application of enzyme-incorporated peptide nanotubes (2005) Bioconjug Chem, 16, pp. 1484-1487Zhao, Z., Banerjee, I.A., Matsui, H., Simultaneous targeted immobilization of anti-human IgG-coated nanotubes and anti-mouse IgG-coated nanotubes on the complementary antigen-atterned surfaces via biological molecular recognition (2005) J Am Chem Soc, 127, pp. 8930-8931Zhang, S., Vauthey, S., Surfactant Peptide Nanostructures, and Uses Thereof, , WO Patent 200303006043A1Zhao, X., Pan, F., Xu, H., Yaseen, M., Shan, H., Hauser, C.A.E., Molecular self-assembly and applications of designer peptide amphiphiles (2010) Chem Soc Rev, 39, pp. 3480-3498Zhou, Y., Patenting activity in synthesis of lipid nanotubes and peptide nanotubes (2007) Recent Pat Nanotechnol, 1, pp. 21-28